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<p>Foodborne pathogens, such as <i>Escherichia coli</i> O157:H7, pose a significant global health threat, necessitating the development of rapid and decentralized detection methods. Conventional laboratory assays often require extensive sample processing and sophisticated instrumentation, which limits their use for on-site, point-of-care testing. This work addresses the need for a simplified and accelerated platform. A rapid bi-functional linker-based immunoassay for the detection of <i>Escherichia coli</i> O157:H7 was developed by accelerating gold nanoparticle (AuNP) aggregation and sedimentation. The bi-functional linker facilitated antigen–antibody binding and controlled AuNP aggregation, while a 1-min centrifugation step expedited sedimentation, significantly shortening the overall assay time. Under these conditions, the assay produced an instrument-free, eye-readable visual response based on the range exhibiting a visible color change (REVC). A portable hand-powered centrifuge was fabricated and applied to detect <i>E. coli</i> O157:H7 in spiked tomato samples, achieving a decision level of 10<sup>3</sup>&#xa0;CFU/25&#xa0;g within a total assay time of just 70&#xa0;min. Compared with passive settling (≈180&#xa0;min), the proposed system significantly reduced detection time while maintaining consistent signal patterns, demonstrating its potential as a rapid, field-deployable biosensor for on-site foodborne pathogen detection.</p>

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A Portable Device for Accelerated Agglomeration and Sedimentation of AuNPs for Rapid Pathogen Detection Via a Bi-functional Linker-Based Immunoassay

  • 期刊论文

  • 发表日期：

    2026年1月7日

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  • Seung Hwan Ham,
  • Hyebin Han,
  • Jungwoo Hahn,
  • Young Jin Choi

摘要

Foodborne pathogens, such as Escherichia coli O157:H7, pose a significant global health threat, necessitating the development of rapid and decentralized detection methods. Conventional laboratory assays often require extensive sample processing and sophisticated instrumentation, which limits their use for on-site, point-of-care testing. This work addresses the need for a simplified and accelerated platform. A rapid bi-functional linker-based immunoassay for the detection of Escherichia coli O157:H7 was developed by accelerating gold nanoparticle (AuNP) aggregation and sedimentation. The bi-functional linker facilitated antigen–antibody binding and controlled AuNP aggregation, while a 1-min centrifugation step expedited sedimentation, significantly shortening the overall assay time. Under these conditions, the assay produced an instrument-free, eye-readable visual response based on the range exhibiting a visible color change (REVC). A portable hand-powered centrifuge was fabricated and applied to detect E. coli O157:H7 in spiked tomato samples, achieving a decision level of 103 CFU/25 g within a total assay time of just 70 min. Compared with passive settling (≈180 min), the proposed system significantly reduced detection time while maintaining consistent signal patterns, demonstrating its potential as a rapid, field-deployable biosensor for on-site foodborne pathogen detection.