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<p><i>B</i><i>urkholderia gladioli</i> pv. <i>cocovenenans</i> is an emerging foodborne pathogen commonly found in foodstuffs such as mushrooms and rice noodles. It produces bongkrekic acid, a highly lethal and thermostable toxin, posing a serious threat to food safety. Therefore, the development of rapid, cost-effective, and highly sensitive detection methods is urgently needed. In this study, Si–OH magnetic beads were employed for nucleic acid extraction, followed by loop-mediated isothermal amplification (LAMP) for the amplification of target DNA. This method effectively distinguishes <i>B. gladioli</i> pv. <i>cocovenenan</i>s from non-toxic <i>B. gladioli</i> strains. The LAMP products were detected using fluorescence signals and visualized through colorimetric analysis, assisted by color analysis software integrated into a smartphone application. Results were classified based on hue, saturation, and lightness (HSL) values: orange (HSL ≦ 44.1) indicated a negative result, while green (HSL ≧ 63.8) indicated a positive result. The entire process can be completed in under 50 min. The detection sensitivity reached 2.5 pg/μL for purified pathogen DNA and 6.68 × 10<sup>2</sup> CFU/25 g for artificially contaminated rice noodle samples. This method is 10 times more sensitive than conventional polymerase chain reaction (PCR), providing a robust and efficient platform for the rapid detection of <i>B. gladioli</i> pv. <i>cocovenenans</i> in food safety monitoring.</p> Graphical Abstract <p></p>

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Colorimetric-LAMP-Based Visual Intelligent Detection for Rapid Identification of Burkholderia gladioli pv. cocovenenans

  • 期刊论文

  • 发表日期：

    2025年9月23日

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  • Lingyan Zheng,
  • Dongmei Li,
  • Jiali Wang,
  • Yumin Shen,
  • Pengcheng Zhang,
  • Hong Duan,
  • Ke Xiong,
  • Joseph Irudayaraj

摘要

Burkholderia gladioli pv. cocovenenans is an emerging foodborne pathogen commonly found in foodstuffs such as mushrooms and rice noodles. It produces bongkrekic acid, a highly lethal and thermostable toxin, posing a serious threat to food safety. Therefore, the development of rapid, cost-effective, and highly sensitive detection methods is urgently needed. In this study, Si–OH magnetic beads were employed for nucleic acid extraction, followed by loop-mediated isothermal amplification (LAMP) for the amplification of target DNA. This method effectively distinguishes B. gladioli pv. cocovenenans from non-toxic B. gladioli strains. The LAMP products were detected using fluorescence signals and visualized through colorimetric analysis, assisted by color analysis software integrated into a smartphone application. Results were classified based on hue, saturation, and lightness (HSL) values: orange (HSL ≦ 44.1) indicated a negative result, while green (HSL ≧ 63.8) indicated a positive result. The entire process can be completed in under 50 min. The detection sensitivity reached 2.5 pg/μL for purified pathogen DNA and 6.68 × 102 CFU/25 g for artificially contaminated rice noodle samples. This method is 10 times more sensitive than conventional polymerase chain reaction (PCR), providing a robust and efficient platform for the rapid detection of B. gladioli pv. cocovenenans in food safety monitoring.

Graphical Abstract