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<p>Traditional fluorescent immunosensors usually suffer from high background signals in food samples, which significantly reduces their detection sensitivity. In this study, δ-iron oxyhydroxide (δ-FeOOH) nanosheets with anti-bacitracin zinc (BAC) monoclonal antibodies (mAb) were conjugated as fluorescence receptors, while amino-functionalized carbon quantum dots (CQDs) as fluorescence donors for BAC. A novel fluorescence immunoassay was developed that eliminated background noise based on the “turn on” principle. Upon introduction of BAC into the sample, the nano-assembled probe showed an increase in fluorescence recovery, leading to higher fluorescence intensity at elevated BAC concentrations. This strategy exhibited a linear response for BAC concentrations ranging from 5 to 40&#xa0;ng/mL, with a limit of detection (LOD) of 2.04&#xa0;ng/mL. Our findings present a promising immunological approach for the sensitive and selective detection of BAC residues in food.</p>

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A Novel Fluorescence Quenched Immunoassay for Sensitive Detection of Bacitracin Zinc Based on δ-FeOOH Nanosheets and Carbon Quantum Dots

  • 期刊论文

  • 发表日期：

    2025年5月17日

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  • Guanqiong Na,
  • Ping Chen,
  • Yanya Qian,
  • Danqun Meng,
  • Zhipeng Liu,
  • Yujuan Shan

摘要

Traditional fluorescent immunosensors usually suffer from high background signals in food samples, which significantly reduces their detection sensitivity. In this study, δ-iron oxyhydroxide (δ-FeOOH) nanosheets with anti-bacitracin zinc (BAC) monoclonal antibodies (mAb) were conjugated as fluorescence receptors, while amino-functionalized carbon quantum dots (CQDs) as fluorescence donors for BAC. A novel fluorescence immunoassay was developed that eliminated background noise based on the “turn on” principle. Upon introduction of BAC into the sample, the nano-assembled probe showed an increase in fluorescence recovery, leading to higher fluorescence intensity at elevated BAC concentrations. This strategy exhibited a linear response for BAC concentrations ranging from 5 to 40 ng/mL, with a limit of detection (LOD) of 2.04 ng/mL. Our findings present a promising immunological approach for the sensitive and selective detection of BAC residues in food.