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<p>Zeaxanthin is a xanthophyll produced by plants, algae, and microorganisms. Its production by bacteria is a rapidly expanding field, as consumers shift their demand from synthetic to natural products and sustainable production methods. In this study, the potential of eucalyptus enzymatic hydrolysate and corn steep liquor (CSL) were evaluated as substrates for the production of zeaxanthin by <i>Flavobacterium</i> sp. To be used as fermentation media, strategies such as the use of surfactants and the supplementation with xylanases were evaluated for the preparation of the eucalyptus hydrolysates to enhance the concentration of glucose and xylose as fermentable sugars. The selected hydrolysis condition for eucalyptus hydrolysate preparation was employing a cellulase to xylanase (C:X) ratio of 2:1 with PEG6000 supplementation, enabling a 33% reduction in cellulase by xylanase supplementation without affecting glucose and xylose yields (52% and 68%, respectively). Microbial fermentation in bioreactor of eucalyptus hydrolysate supplemented with CSL resulted in a zeaxanthin and total carotenoids concentration of 0.60&#xa0;mg/L and 0.97&#xa0;mg/L, respectively. Thus, CSL supplementation under controlled bioreactor conditions increased zeaxanthin yield from 0.032&#xa0;mg/g in flasks to 0.096&#xa0;mg/g, highlighting its potential as an economical strategy to improve efficiency. This study contributes to the valorization of eucalyptus residues as cost-effective sources to produce high valuable compounds with biotechnological applications.</p>

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Sustainable Zeaxanthin Production: Evaluating Eucalyptus Hydrolysate as a Fermentation Substrate by an Antarctic Flavobacterium Sp

  • 期刊论文

  • 发表日期：

    2025年12月6日

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  • Bianca Decarlini,
  • Florencia Cebreiros,
  • Eugenia Vila

摘要

Zeaxanthin is a xanthophyll produced by plants, algae, and microorganisms. Its production by bacteria is a rapidly expanding field, as consumers shift their demand from synthetic to natural products and sustainable production methods. In this study, the potential of eucalyptus enzymatic hydrolysate and corn steep liquor (CSL) were evaluated as substrates for the production of zeaxanthin by Flavobacterium sp. To be used as fermentation media, strategies such as the use of surfactants and the supplementation with xylanases were evaluated for the preparation of the eucalyptus hydrolysates to enhance the concentration of glucose and xylose as fermentable sugars. The selected hydrolysis condition for eucalyptus hydrolysate preparation was employing a cellulase to xylanase (C:X) ratio of 2:1 with PEG6000 supplementation, enabling a 33% reduction in cellulase by xylanase supplementation without affecting glucose and xylose yields (52% and 68%, respectively). Microbial fermentation in bioreactor of eucalyptus hydrolysate supplemented with CSL resulted in a zeaxanthin and total carotenoids concentration of 0.60 mg/L and 0.97 mg/L, respectively. Thus, CSL supplementation under controlled bioreactor conditions increased zeaxanthin yield from 0.032 mg/g in flasks to 0.096 mg/g, highlighting its potential as an economical strategy to improve efficiency. This study contributes to the valorization of eucalyptus residues as cost-effective sources to produce high valuable compounds with biotechnological applications.