<p>Infertility as a stressful event has an adverse effect on individuals’ negative emotions. Previous studies have demonstrated a relationship between stress dyadic coping and negative emotions but have focused mainly on one spouse not both partners. The present study used a dyadic approach to explore the relationship between perceived stress and negative emotions and the potential mediating effect of dyadic coping on the relationship between perceived stress and negative emotions.&#xa0;From December 2022 toMay 2023 276 couples who were undergoing in vitro fertilization(IVF) were recruited for this study. Participants were assessed using the Hospital Anxiety and Depression Scale, the Perceived Stress Scale, and the Dyadic Coping Inventory. The actor-partner interdependence mediation model (APIMeM) was utilized to examine data from the dyadic relationships.&#xa0;Wives’ positive dyadic coping (<i>β</i> = 0.0424, 95% <i>CI</i> = 0.0116 ~ 0.0807) had a significant relative mediating effect on the influence of perceived stress on negative emotions, and it played a partial mediating role, accounting for 7.51%. Wives’ (<i>β</i> = 0.0521, 95% <i>CI</i> = 0.0195 ~ 0.0923) and husbands’ (<i>β</i> = 0.0601, 95% <i>CI</i> = 0.0229 ~ 0.1045) negative dyadic coping styles had significant relative mediating effects on the influence of perceived stress on negative emotions, and they played partial mediating roles, accounting for 9.23% and 10.13%, respectively. However, it was found that the perceived stress of wives with infertility positively influenced husbands’ negative emotions only via wives’ negative dyadic coping (<i>β</i> = 0.0303, 95% <i>CI</i> = 0.0034 ~ 0.0606).&#xa0;These findings suggest that interventions for couples undergoing IVF treatment aimed at improving dyadic coping could help mitigate the negative impact of high perceived stress on their negative emotions.</p>