<p>Trust is a fundamental component of social relationships and a prerequisite for smooth and efficient interactions. However, trust is fragile and easily threatened or broken. Recovering trust as quickly and effectively as possible is a top priority. The current study conducts three experiments (<i>N</i> = 1036) to examine how individuals’ moral conscience influenced trust violation and its repair strategies under virtual and real scenarios. In Experiment 1 we aimed to examine the effect of state-based conscience on trust violation, and we found conscience elicited trusting behavior after trust violation. In Experiment 2 we aimed to disclose the impact of trait-based conscience on trust violation in real-world and virtual settings, and we found participants with low conscience levels perceived more serious breaches of trust than those with high levels in the real scenario, only participants with high conscience levels were more likely to trust others, regardless of whether in the virtual or real scenarios. In Experiment 3 we aimed to examine the effect of the influence of conscience on preferences for trust repair strategies in real-world and virtual settings, and we found participants favored the financial compensation strategy in the real scenario but preferred the apology strategy in the virtual scenario, however, participants with high conscience levels took more effective trust repair strategies and thus had a more positive effect on trust recovery after violations. Together, results indicated that moral conscience mattered: higher conscience level diminished the breaches of trust violation and made trust restoration easier, and conscience could predict the effectiveness of different restoration strategies in real scenario.</p>