<p>The rise of anti-immigrant sentiment and growth of occupational precarity from automation and globalization suggest anxiety over job loss is contributing to mass support for exclusionary immigration policy in the twenty-first century. However, since most research has largely focused on populations in the United States and Europe, it is unclear whether this anxiety is fueling the recent shift toward exclusionary policy in the Global South and North. Accordingly, this study evaluates whether support for exclusionary immigration policy amongst workers employed in insecure jobs in the Global North and South is fueled by perceptions of immigrant threat. We argue this consideration is important because threat-based support amongst insecure workers is amplified by the degree of economic inequality in countries. Drawing on worker-level data from the most recent wave of the World Value Survey, we estimate conditional process models of support for exclusionary immigration policy amongst 33,590 workers in 42 countries to test whether the association between occupational insecurity and support for exclusionary policy is mediated by threat perception in both developed and less-developed countries. We find employment in jobs at risk of replacement by automation is associated with greater threat perception which, in turn, increases support for exclusionary policy. Moreover, we find this process is amplified at moderate to high levels of national income inequality. This highlights the importance of understanding the rising mass support for exclusionary immigration policy in the Global North and South as the consequence of occupational precarity in countries where economic resources are unequally distributed.</p>