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<p>Sepsis in young infants can lead to high mortality and morbidity, and its nonspecific signs require early and on-site detection of infections. The most common cause of infections in early-onset sepsis is <i>Escherichia coli</i>, particularly in premature and low-birth-weight infants. Here, the authors developed a rapid method based on loop-mediated isothermal amplification (LAMP) assay for detecting <i>E. coli</i> in newborn blood samples. The results showed detection of <i>E. coli</i> with a limit of detection (LOD) of 70 CFU/mL, confirming the assay’s reliability in distinguishing positive and negative cases compared to PCR-based detection. This LAMP assay uses 10 µL of blood and takes less than 1&#xa0;h. A total of 66 blood samples from young infants were used to validate the LAMP assay, which demonstrated excellent sensitivity and specificity. A rapid and easy LAMP assay was successfully validated with young infants’ blood samples, demonstrating excellent potential for on-site detection of <i>E. coli</i>.</p>

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Evaluation and Validation of a Loop-Mediated Isothermal Amplification (LAMP) Assay for Detection of Escherichia coli from Blood Samples of Young Infants with Sepsis

  • 期刊论文

  • 发表日期：

    2026年4月10日

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  • Shukla Banerjee,
  • H. K. Mithun,
  • Rajesh P. Shastry

摘要

Sepsis in young infants can lead to high mortality and morbidity, and its nonspecific signs require early and on-site detection of infections. The most common cause of infections in early-onset sepsis is Escherichia coli, particularly in premature and low-birth-weight infants. Here, the authors developed a rapid method based on loop-mediated isothermal amplification (LAMP) assay for detecting E. coli in newborn blood samples. The results showed detection of E. coli with a limit of detection (LOD) of 70 CFU/mL, confirming the assay’s reliability in distinguishing positive and negative cases compared to PCR-based detection. This LAMP assay uses 10 µL of blood and takes less than 1 h. A total of 66 blood samples from young infants were used to validate the LAMP assay, which demonstrated excellent sensitivity and specificity. A rapid and easy LAMP assay was successfully validated with young infants’ blood samples, demonstrating excellent potential for on-site detection of E. coli.