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<p>In plants, pathogenic infection causes oxidative damage to the host plants and eventually leads to the destruction of the plant’s metabolism due to the oxidation of essential biomolecules. In this investigation, we monitored the effects of fusarium wilt and damping-off mediated generation of Reactive oxygen species (ROS) and their effective disposal by the antioxidative defence system in tomato plants. The main aim of the current research work was to assess the seed priming remedial effects of <i>Trichoderma virens</i> (Tv) (1 × 10<sup>7</sup> spores/mL) as a biocontrol agent and jasmonic acid (JA) (1&#xa0;μM) as a chemical inducer against the pathogen- induced oxidative stress in diseased plants. Thirty days old primed and unprimed tomato seedlings were inoculated with <i>Fusarium oxysporum</i> f. sp. <i>lycopersici</i> (Fol) (1 × 10<sup>6</sup> spores/mL) and <i>Rhizoctonia solani</i> (Rs) (2% w/v) in a different set of treatments, and after thirty days of pathogen inoculation, leaf sampling was done for twelve treatments randomly to record various physiochemical parameters. A significant increase in the oxidative stress markers measured in terms of MDA (Malondialdehyde) and H<sub>2</sub>O<sub>2</sub> contents was observed in <i>Fol</i> and Rs-infected tomato plants compared with non-inoculated ones. Moreover, pre-treatment of tomato seeds with ameliorative agents lowered the extent of oxidative damage in the case of both pathogens. Consequently, to combat these ROS in comparison to the control, a marked enhancement in the antioxidative defence system was observed, as evidenced by increased ascorbic acid (AsA), tocopherol, glutathione (GSH), phenolic, and flavonoid contents, and hence, free radical scavenging activity. This physicochemical analysis further revealed that seed priming with <i>T. virens</i> and JA in combination in pathogenically stressed plants in the treatments, T8 (Fol + Tv + JA) and T12 (Rs + Tv + JA) has the most remedial influences when compared with non- primed pathogen-inoculated plants under the treatments, T5 (Fol) and T9 (Rs). These findings support the priming-induced disease suppressive influences of <i>T. virens</i> and JA on tomato plants through the enhanced activation of antioxidative defence mechanisms against Fusarium wilt and damping off diseases.</p>

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Antioxidative Defence Mechanisms in Tomato Plants Induced by Biopriming with Trichoderma virens and Jasmonic Acid Against Fusarium Wilt and Damping-Off

  • 期刊论文

  • 发表日期：

    2026年8月3日

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  • Monika Sood,
  • Vipul Kumar,
  • Sarvjeet Kukreja

摘要

In plants, pathogenic infection causes oxidative damage to the host plants and eventually leads to the destruction of the plant’s metabolism due to the oxidation of essential biomolecules. In this investigation, we monitored the effects of fusarium wilt and damping-off mediated generation of Reactive oxygen species (ROS) and their effective disposal by the antioxidative defence system in tomato plants. The main aim of the current research work was to assess the seed priming remedial effects of Trichoderma virens (Tv) (1 × 107 spores/mL) as a biocontrol agent and jasmonic acid (JA) (1 μM) as a chemical inducer against the pathogen- induced oxidative stress in diseased plants. Thirty days old primed and unprimed tomato seedlings were inoculated with Fusarium oxysporum f. sp. lycopersici (Fol) (1 × 106 spores/mL) and Rhizoctonia solani (Rs) (2% w/v) in a different set of treatments, and after thirty days of pathogen inoculation, leaf sampling was done for twelve treatments randomly to record various physiochemical parameters. A significant increase in the oxidative stress markers measured in terms of MDA (Malondialdehyde) and H2O2 contents was observed in Fol and Rs-infected tomato plants compared with non-inoculated ones. Moreover, pre-treatment of tomato seeds with ameliorative agents lowered the extent of oxidative damage in the case of both pathogens. Consequently, to combat these ROS in comparison to the control, a marked enhancement in the antioxidative defence system was observed, as evidenced by increased ascorbic acid (AsA), tocopherol, glutathione (GSH), phenolic, and flavonoid contents, and hence, free radical scavenging activity. This physicochemical analysis further revealed that seed priming with T. virens and JA in combination in pathogenically stressed plants in the treatments, T8 (Fol + Tv + JA) and T12 (Rs + Tv + JA) has the most remedial influences when compared with non- primed pathogen-inoculated plants under the treatments, T5 (Fol) and T9 (Rs). These findings support the priming-induced disease suppressive influences of T. virens and JA on tomato plants through the enhanced activation of antioxidative defence mechanisms against Fusarium wilt and damping off diseases.