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<p>The sorption of cells of an electrogenic strain of microorganisms <i>Micrococcus luteus</i> 1-i onto the surface of carbon fabric polarized by applying a voltage of 1&#xa0;V was studied. In the absence of voltage, the sorption of <i>M. luteus</i> cells on the carbon fabric was observed to be 36.5%. The sorption of <i>M. luteus</i> cells, pre-killed by heating under similar conditions, was notably lower, at 26.5%. This value remained almost the same when the voltage was increased to 1&#xa0;V. Therefore, the specified voltage value had no adverse effect on the adhesion processes of microbial cells on the electrode surface. The determination of microbial cell viability was carried out using luminescence microscopy, a Luna-II YF cell counter, and double staining of cells with fluorescent dyes, specifically acridine orange and propidium iodide. The viability of microorganisms sorbed on carbon fabric at a voltage of 1&#xa0;V was maintained at a level comparable to that of the control (0&#xa0;V). Furthermore, the viability of the bacterial suspension remained the same following contact with the polarized electrodes (1&#xa0;V). It was demonstrated that the voltage generated in a microbial fuel cell (MFC) exhibited no detrimental impact on the viability of <i>Micrococcus luteus</i> strain cells, whether suspended in the interelectrode space or adsorbed onto the electrodes. Furthermore, the investigated microorganisms remained viable across a broad pH range of the medium, from 4.0 to 12.0. The obtained results illustrate the remarkable resilience of the <i>Micrococcus luteus</i> 1-i bacterial strain to the harsh environmental conditions that are inherent to the functioning of MFCs.</p>

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Sorption of Microbial Electrogenic Bacterium Strain Micrococcus luteus on Charged Carbon Surface

  • 期刊论文

  • 发表日期：

    2025年5月9日

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  • Michael N. Saksonov,
  • Devard I. Stom,
  • Ksenia A. Khramtsova,
  • Galina O. Zhdanova,
  • Aleksander B. Kupchinsky,
  • Sergey V. Alferov,
  • Qixing Zhou,
  • Lepakshi Barbora

摘要

The sorption of cells of an electrogenic strain of microorganisms Micrococcus luteus 1-i onto the surface of carbon fabric polarized by applying a voltage of 1 V was studied. In the absence of voltage, the sorption of M. luteus cells on the carbon fabric was observed to be 36.5%. The sorption of M. luteus cells, pre-killed by heating under similar conditions, was notably lower, at 26.5%. This value remained almost the same when the voltage was increased to 1 V. Therefore, the specified voltage value had no adverse effect on the adhesion processes of microbial cells on the electrode surface. The determination of microbial cell viability was carried out using luminescence microscopy, a Luna-II YF cell counter, and double staining of cells with fluorescent dyes, specifically acridine orange and propidium iodide. The viability of microorganisms sorbed on carbon fabric at a voltage of 1 V was maintained at a level comparable to that of the control (0 V). Furthermore, the viability of the bacterial suspension remained the same following contact with the polarized electrodes (1 V). It was demonstrated that the voltage generated in a microbial fuel cell (MFC) exhibited no detrimental impact on the viability of Micrococcus luteus strain cells, whether suspended in the interelectrode space or adsorbed onto the electrodes. Furthermore, the investigated microorganisms remained viable across a broad pH range of the medium, from 4.0 to 12.0. The obtained results illustrate the remarkable resilience of the Micrococcus luteus 1-i bacterial strain to the harsh environmental conditions that are inherent to the functioning of MFCs.