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<p>‘Green’ synthesis of nanoparticles from microorganisms and plants is a sustainable alternative to the conventional chemo-physical methods because of its environment-friendliness. Microorganisms take up extracellular and intracellular pathways to manufacture nanoparticles. This study was aimed at optimization of the parameters influencing the biosynthesis of Cu/CuONPs (Copper/Copper oxide nanoparticles) by <i>Pantoea agglomerans</i> CPHN2 using OFAT (One-factor-at-a-time) approach. The optimum biotransformation was achieved at 5&#xa0;mM concentration of CuSO<sub>4</sub>, 32&#xa0;h incubation time, 6&#xa0;h reaction time, equal mixing ratio of supernatant and CuSO<sub>4</sub>, 7 pH, and 23&#xa0;°C temperature. Biosynthesized Cu/CuONPs along with those formed chemically, were characterized using UV–Visible spectrophotometer, resulting in a characteristic peak between 550 and 650&#xa0;nm. Dynamic light scattering (DLS) reported that Z-average and Zeta potential of Cu/CuONPs were 291.9&#xa0;nm and − 21&#xa0;mV for biosynthesized and 179&#xa0;nm and − 6.49&#xa0;mV for chemosynthesized NPs. HR-TEM (High Resolution-Transmission Electron Microscopy) revealed hexagonal NPs in size range of 5–35&#xa0;nm. In addition, FTIR (Fourier Transform Infrared) spectrum elucidated different peaks attributed to the Cu/CuONPs, alkynes, O–H, and N–H bonds, of the molecules involved in synthesis and stabilization of biosynthesized NPs. Antimicrobial assay conducted in the presence of 100&#xa0;µg/ml solution of biosynthesized Cu/CuONPs led to 66.01, 68.31, and 55.73%, inhibition of <i>Staphylococcus aureus</i> growth at 7, 14, and 21&#xa0;h, respectively, whereas chemosynthesized Cu/CuONPs had negligible impact. The biosynthesized NPs also exhibited 5.48-fold increase in inhibitory activity than chemosynthesized NPs on <i>Bacillus subtilis</i> growth. The results suggests that biosynthesized Cu/CuONPs can serve as ecofriendly and economical method for managing the infections led by gram-positive bacteria.</p> Graphical Abstract <p></p>

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Optimization, Characterization and Antibacterial Activity of Copper Nanoparticles Biosynthesized Using Pantoea agglomerans CPHN2

  • 期刊论文

  • 发表日期：

    2025年2月8日

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  • Simran Rani,
  • Pradeep Kumar,
  • Priyanka Dahiya,
  • Aditi Mehta,
  • Amita Suneja Dang,
  • Pooja Suneja

摘要

‘Green’ synthesis of nanoparticles from microorganisms and plants is a sustainable alternative to the conventional chemo-physical methods because of its environment-friendliness. Microorganisms take up extracellular and intracellular pathways to manufacture nanoparticles. This study was aimed at optimization of the parameters influencing the biosynthesis of Cu/CuONPs (Copper/Copper oxide nanoparticles) by Pantoea agglomerans CPHN2 using OFAT (One-factor-at-a-time) approach. The optimum biotransformation was achieved at 5 mM concentration of CuSO4, 32 h incubation time, 6 h reaction time, equal mixing ratio of supernatant and CuSO4, 7 pH, and 23 °C temperature. Biosynthesized Cu/CuONPs along with those formed chemically, were characterized using UV–Visible spectrophotometer, resulting in a characteristic peak between 550 and 650 nm. Dynamic light scattering (DLS) reported that Z-average and Zeta potential of Cu/CuONPs were 291.9 nm and − 21 mV for biosynthesized and 179 nm and − 6.49 mV for chemosynthesized NPs. HR-TEM (High Resolution-Transmission Electron Microscopy) revealed hexagonal NPs in size range of 5–35 nm. In addition, FTIR (Fourier Transform Infrared) spectrum elucidated different peaks attributed to the Cu/CuONPs, alkynes, O–H, and N–H bonds, of the molecules involved in synthesis and stabilization of biosynthesized NPs. Antimicrobial assay conducted in the presence of 100 µg/ml solution of biosynthesized Cu/CuONPs led to 66.01, 68.31, and 55.73%, inhibition of Staphylococcus aureus growth at 7, 14, and 21 h, respectively, whereas chemosynthesized Cu/CuONPs had negligible impact. The biosynthesized NPs also exhibited 5.48-fold increase in inhibitory activity than chemosynthesized NPs on Bacillus subtilis growth. The results suggests that biosynthesized Cu/CuONPs can serve as ecofriendly and economical method for managing the infections led by gram-positive bacteria.

Graphical Abstract