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Aim <p>This study investigates Cell Division Cycle 27 (CDC27) expression in HNSCC, examining its links to patient prognosis, tumor immune infiltration, and its potential as a therapeutic target.</p> Materials and Methods <p>This study exclusively utilized The Cancer Genome Atlas (TCGA-HNSCC) dataset containing 528 cases, which was further supplemented with proteomic data provided by the Clinical Proteomic Tumor Analysis Consortium (CPTAC) along with comprehensive clinical and genomic data. mRNA and protein expression were compared between malignant and matched normal tissues using UALCAN. We then correlated CDC27 levels with clinicopathological features and overall survival by constructing Kaplan–Meier curves and applying Cox proportional hazards models. Additionally, protein-protein interaction networks and functional enrichment analyses were conducted with STRING and Metascape, while the relationship between CDC27 expression and immune cell infiltration was evaluated through correlation analyses using TISIDB databases.</p> Results <p>It was observed that CDC27, mRNA, and protein levels are significantly upregulated in HNSCC compared to normal tissues (<i>p</i> &lt; 0.001). Elevated CDC27 expression correlated with adverse clinicopathological features, including high tumor grade, and increased expression in metastatic tissues. Survival analysis revealed that high CDC27 expression is significantly associated with poorer overall survival in HNSCC patients (<i>p</i> = 0.00465). Furthermore, increased CDC27 levels were linked to an immunosuppressive tumor microenvironment characterized by negative association with CD8 + T cell and B cell infiltration, and positive association with immunosuppressive markers such as TGF-β1 and its receptor.</p> Conclusions <p>CDC27 overexpression in HNSCC correlates with aggressive disease, poorer survival, and an immunosuppressive microenvironment, underscoring its promise as both a prognostic biomarker and therapeutic target. Further studies should clarify CDC27’s mechanisms in tumor progression, treatment resistance, and immune regulation.</p>

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Comprehensive Analysis of Expression, Prognostic and Immune Significance of CDC27 Gene in Head and Neck Cancer

  • 期刊论文

  • 发表日期：

    2025年9月18日

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  • Pallav Sharda,
  • Sarvagya Sharma,
  • Khushali K. Shah

摘要

Aim

This study investigates Cell Division Cycle 27 (CDC27) expression in HNSCC, examining its links to patient prognosis, tumor immune infiltration, and its potential as a therapeutic target.

Materials and Methods

This study exclusively utilized The Cancer Genome Atlas (TCGA-HNSCC) dataset containing 528 cases, which was further supplemented with proteomic data provided by the Clinical Proteomic Tumor Analysis Consortium (CPTAC) along with comprehensive clinical and genomic data. mRNA and protein expression were compared between malignant and matched normal tissues using UALCAN. We then correlated CDC27 levels with clinicopathological features and overall survival by constructing Kaplan–Meier curves and applying Cox proportional hazards models. Additionally, protein-protein interaction networks and functional enrichment analyses were conducted with STRING and Metascape, while the relationship between CDC27 expression and immune cell infiltration was evaluated through correlation analyses using TISIDB databases.

Results

It was observed that CDC27, mRNA, and protein levels are significantly upregulated in HNSCC compared to normal tissues (p < 0.001). Elevated CDC27 expression correlated with adverse clinicopathological features, including high tumor grade, and increased expression in metastatic tissues. Survival analysis revealed that high CDC27 expression is significantly associated with poorer overall survival in HNSCC patients (p = 0.00465). Furthermore, increased CDC27 levels were linked to an immunosuppressive tumor microenvironment characterized by negative association with CD8 + T cell and B cell infiltration, and positive association with immunosuppressive markers such as TGF-β1 and its receptor.

Conclusions

CDC27 overexpression in HNSCC correlates with aggressive disease, poorer survival, and an immunosuppressive microenvironment, underscoring its promise as both a prognostic biomarker and therapeutic target. Further studies should clarify CDC27’s mechanisms in tumor progression, treatment resistance, and immune regulation.