<p>Plant-specific TIFY-transcription factor (TF) family contains four sub-families, Jasmonate ZIM domain (JAZ), TIFY, Peapod (PPD), and ZIM and ZIM-like (ZML), according to their specific domain architectures. JAZ-subfamily being the key repressor of plant Jasmonic Acid (JA) signaling pathway, TIFY-TFs dynamically contribute to plant growth, development, wounding, herbivory, biotic and abiotic stress responses. Among six two-partner fusion events presented here, four events are from JAZ sub-family, JAZ1-CXE1 (Carboxylesterase1) fusion, JAZ3-TDX (Tetratricopeptide repeat containing thioredoxin like protein) fusion, JAZ4-SURF1 (Surfeit locus 1 cytochrome c oxidase biogenesis protein) fusion and Receptor like cytoplasmic kinase (RLCK)-JAZ12 fusion, one is from Peapod subfamily, PPD1-ARD (Acireductone dioxygenase) fusion, and one is from ZML subfamily, ZML1-Ca²⁺transporting ATPase protein fusion. This study attempts validating each TIFY-fusion Protein Report by (i) identification of fusion partners having consecutive gene locations and showing high sequence identity with the fusion protein (FP), (ii) detection of accurate breakpoints and insertion of non-coding-derived sequences in the FPs, (iii) uncovering functional similarities between the fusion partners, which together, may be correlated to the transcriptional read-through-mediated origination of the fusion proteins. Prediction of Intrinsic Disordered Region (IDR) and subcellular localization of TIFY-fusion proteins reveal their nucleus-based localization and persistence of disordered-regions, supporting their probable functionality. Here, 49 TIFY-Fusion protein reports and 10 TIFY-Fusion gene reports are clearly uncovering a evolutionary trend to impart TIFY-TF-multifunctionality through these six two-partner and two three-partner fusion events in eudicots. This study presents new insights into the evolutionary significance of plant TIFY-TF family to improve the adaptive versatility of eudicot plant’s sessile existence.</p>