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<p>Mining key genes involved in the synthesis of flavonoids, carotenoids, and ascorbic acid at different stages of potato tuber development, is of great importance for molecular breeding of potato plants. To this end, transcriptomic data was obtained from three different stages of potato tuber development, including hook-like tip (SI), swollen tip (SII), and beginning of tuberization (SIII). Overall, 3160 genes and 4982 transcripts were identified. A comparative analysis of gene expression across different stages identified 1,137, 2,889, and 956 differentially expressed genes (DEGs) in the SI vs. SII, SI vs. SIII, and SII vs. SIII comparisons, respectively. A higher number of DEGs were associated with flavonoid biosynthesis compared to ascorbic acid and carotenoids. As potato tubers developed from SI to SIII, the levels of ascorbic acid decreased, whereas the levels of carotenoids and flavonoids increased. Additionally, there was a significant increase in the total protein and flavonoid content from SI to SIII. Twelve genes with significant expression associated with the synthesis of metabolites, including <i>ABA2</i>, <i>CCD7</i>, <i>CHY2</i>, <i>AAO</i>, <i>NCED1</i>, <i>CYP98A3</i>, <i>ACT</i>, <i>SAT</i>, <i>BEAT</i>, <i>MPG1</i>, <i>NDE/D</i> and <i>GGP1</i> were selected and analyzed by qRT-PCR. For the majority of genes, there was a significant correlation in expression between the RNA-seq data and the corresponding qRT-PCR data. This study provides a comprehensive view of the expression patterns of key tuber metabolites biosynthetic and regulatory genes, which could be of great benefit for plant breeders to develop potato varieties with enhanced nutritional benefits using both traditional and molecular methods.</p>

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Mining Key Genes Involved in the Synthesis of Flavonoids, Carotenoids, and Ascorbic Acid Metabolites During Potato Tuber Development

  • 期刊论文

  • 发表日期：

    2026年4月21日

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  • Shiva Siahmansour,
  • Farhad Nazarian-Firouzabadi,
  • Ahmad Ismaili,
  • Seyed Sajad Sohrabi

摘要

Mining key genes involved in the synthesis of flavonoids, carotenoids, and ascorbic acid at different stages of potato tuber development, is of great importance for molecular breeding of potato plants. To this end, transcriptomic data was obtained from three different stages of potato tuber development, including hook-like tip (SI), swollen tip (SII), and beginning of tuberization (SIII). Overall, 3160 genes and 4982 transcripts were identified. A comparative analysis of gene expression across different stages identified 1,137, 2,889, and 956 differentially expressed genes (DEGs) in the SI vs. SII, SI vs. SIII, and SII vs. SIII comparisons, respectively. A higher number of DEGs were associated with flavonoid biosynthesis compared to ascorbic acid and carotenoids. As potato tubers developed from SI to SIII, the levels of ascorbic acid decreased, whereas the levels of carotenoids and flavonoids increased. Additionally, there was a significant increase in the total protein and flavonoid content from SI to SIII. Twelve genes with significant expression associated with the synthesis of metabolites, including ABA2, CCD7, CHY2, AAO, NCED1, CYP98A3, ACT, SAT, BEAT, MPG1, NDE/D and GGP1 were selected and analyzed by qRT-PCR. For the majority of genes, there was a significant correlation in expression between the RNA-seq data and the corresponding qRT-PCR data. This study provides a comprehensive view of the expression patterns of key tuber metabolites biosynthetic and regulatory genes, which could be of great benefit for plant breeders to develop potato varieties with enhanced nutritional benefits using both traditional and molecular methods.