Betv1 plays an important regulatory role in plant stress resistance, the purpose of this study was to understand the characteristics and potential functions of Betv1 gene family in industrial hemp genome, and to provide important clues for further study of the regulation of Betv1 gene on drought stress and hormones. According to the reference sequence in the transcriptome data of industrial hemp in the laboratory, the Betv1 gene family of industrial hemp was identified by bioinformatics method, and the physicochemical characteristics, gene structure, promoter cis-acting elements were systematically analyzed, and the expression pattern of drought stress and abscisic acid (ABA), methyl-jasmonate (MeJA), salicylic acid (SA), and jasmonic acid (JA) hormones were determined. The results indicated that a total of 11 Betv1 genes were selected from the industrial hemp Betv1 gene family of industrial hemp, which were located on 3 chromosomes, and belonged to the three subfamilies the coding gene contained 152 ~ 187 amino acids (aa), the theoretical isoelectric points (pI) was from 4.76 ~ 6.43; Analysis of protein conserved motifs revealed 10 conserved motifs in Betv1, among them, Motif1, Motif2 and Motif4 were shared by 11 members; Analysis of the promoter regulatory elements revealed that the promoter region of Betv1 family members was rich in multiple stress responses and phytohormone-responsive elements such as ABA, MeJA, SA, and JA, etc. Quantitative real-time analysis showed no significant difference between CsMLP4 and CsMLP8 gene expression during the drought prophase but significant upregulation in the late phase. The expression of CsMLP4, CsMLP5, and CsMLP6 genes were significantly increased in the SA treatment groups. In the MeJA-treated group, the CsMLP4 expression level was significantly increased. The expression levels of CsMLP5 and CsMLP8 genes were suppressed under gibberellin (GA) treatment. Gene expression of CsMLP4, CsMLP5, CsMLP6, and CsMLP8 was upregulated in the ABA treated group, which was not significant compared with the control group. Overexpression of CsMLP8 confers greater drought tolerance in Arabidopsis than in wild-type plants. Research shows that the evolutionary structure of Betv1 gene family members has high conservation and can respond to exogenous hormones and drought stress. The CsMLP8 gene could thus be utilized to improve plant tolerance to drought stress.

Identification and Expression Analysis of the Betv1 Gene Family in Industrial Hemp

期刊论文
发表日期：

2026年2月24日

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Qingyi Shao,
Xinyue Wang,
Xuesong Wang,
Sen Yang,
Jiaang Cao,
Nan Zhao,
Qiang Wang,
Hexuan Xu,
Lijie Liu

摘要

Betv1 plays an important regulatory role in plant stress resistance, the purpose of this study was to understand the characteristics and potential functions of Betv1 gene family in industrial hemp genome, and to provide important clues for further study of the regulation of Betv1 gene on drought stress and hormones. According to the reference sequence in the transcriptome data of industrial hemp in the laboratory, the Betv1 gene family of industrial hemp was identified by bioinformatics method, and the physicochemical characteristics, gene structure, promoter cis-acting elements were systematically analyzed, and the expression pattern of drought stress and abscisic acid (ABA), methyl-jasmonate (MeJA), salicylic acid (SA), and jasmonic acid (JA) hormones were determined. The results indicated that a total of 11 Betv1 genes were selected from the industrial hemp Betv1 gene family of industrial hemp, which were located on 3 chromosomes, and belonged to the three subfamilies the coding gene contained 152 ~ 187 amino acids (aa), the theoretical isoelectric points (pI) was from 4.76 ~ 6.43; Analysis of protein conserved motifs revealed 10 conserved motifs in Betv1, among them, Motif1, Motif2 and Motif4 were shared by 11 members; Analysis of the promoter regulatory elements revealed that the promoter region of Betv1 family members was rich in multiple stress responses and phytohormone-responsive elements such as ABA, MeJA, SA, and JA, etc. Quantitative real-time analysis showed no significant difference between CsMLP4 and CsMLP8 gene expression during the drought prophase but significant upregulation in the late phase. The expression of CsMLP4, CsMLP5, and CsMLP6 genes were significantly increased in the SA treatment groups. In the MeJA-treated group, the CsMLP4 expression level was significantly increased. The expression levels of CsMLP5 and CsMLP8 genes were suppressed under gibberellin (GA) treatment. Gene expression of CsMLP4, CsMLP5, CsMLP6, and CsMLP8 was upregulated in the ABA treated group, which was not significant compared with the control group. Overexpression of CsMLP8 confers greater drought tolerance in Arabidopsis than in wild-type plants. Research shows that the evolutionary structure of Betv1 gene family members has high conservation and can respond to exogenous hormones and drought stress. The CsMLP8 gene could thus be utilized to improve plant tolerance to drought stress.