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<p>Aquatic invertebrates, particularly crustaceans like <i>Daphnia</i>, are extensively used as model organisms for assessing the ecotoxicological effects of endocrine-disrupting chemicals (EDCs), including bisphenols. These organisms provide critical insights for regulatory agencies in categorizing such chemicals. However, a significant limitation lies in the lack of understanding of the molecular mechanisms within cells and specific tissue responses to toxicants. Concurrently, there is a global paradigm shift toward utilizing <i>in vitro</i> platforms for toxicity assessments. Recognizing the absence of established crustacean cell culture systems, we developed a primary embryonic cell culture platform from <i>Daphnia magna</i> to investigate the cellular and molecular effects of bisphenol A (BPA) exposure. The lethal concentration (LC<sub>50</sub>) values derived from <i>in vitro</i> studies were used to design <i>in vivo</i> exposures, and real-time PCR analysis was conducted to compare gene expression patterns for selected genes (<i>cat</i>, <i>hsp70</i>, <i>vtg1</i>, and <i>cyp4</i>). While these genes exhibited similar patterns of upregulation and downregulation in both <i>in vitro</i> and <i>in vivo</i> systems, the fold change between the two methods was statistically significant. This validation highlights the reliability and relevance of the developed <i>in vitro</i> platform for exploring mechanistic toxicological effects. Our findings underscore the potential of <i>D. magna in vitro</i> platforms to provide valuable mechanistic insights into EDC toxicity and emphasize the importance of integrating <i>in vitro</i> and <i>in vivo</i> approaches for a comprehensive understanding of toxicant impacts.</p> Graphical abstract <p></p>

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Investigating the translational effects of in vitro LC50 on in vivo responses: Comparative toxicity analysis of bisphenol A (BPA) in Daphnia magna

  • 期刊论文

  • 发表日期：

    2025年10月30日

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  • C P Sreevidya,
  • V Ajitha,
  • T M Manoj Kumar,
  • S Manomi,
  • M A Anjaly,
  • I S Bright Singh,
  • Jayesh Puthumana

摘要

Aquatic invertebrates, particularly crustaceans like Daphnia, are extensively used as model organisms for assessing the ecotoxicological effects of endocrine-disrupting chemicals (EDCs), including bisphenols. These organisms provide critical insights for regulatory agencies in categorizing such chemicals. However, a significant limitation lies in the lack of understanding of the molecular mechanisms within cells and specific tissue responses to toxicants. Concurrently, there is a global paradigm shift toward utilizing in vitro platforms for toxicity assessments. Recognizing the absence of established crustacean cell culture systems, we developed a primary embryonic cell culture platform from Daphnia magna to investigate the cellular and molecular effects of bisphenol A (BPA) exposure. The lethal concentration (LC50) values derived from in vitro studies were used to design in vivo exposures, and real-time PCR analysis was conducted to compare gene expression patterns for selected genes (cat, hsp70, vtg1, and cyp4). While these genes exhibited similar patterns of upregulation and downregulation in both in vitro and in vivo systems, the fold change between the two methods was statistically significant. This validation highlights the reliability and relevance of the developed in vitro platform for exploring mechanistic toxicological effects. Our findings underscore the potential of D. magna in vitro platforms to provide valuable mechanistic insights into EDC toxicity and emphasize the importance of integrating in vitro and in vivo approaches for a comprehensive understanding of toxicant impacts.

Graphical abstract