Synthesis, characterization, in vitro anti-stress, anti-obesity and anti-mitotic activity of benzaldehyde cyanoacetyl hydrazone derivative
摘要
Aromatic heterocycles are the basis for numerous agrochemical and medicinal chemicals, as well as a variety of molecules found in life. In this study, cyanoacetyl hydrazone compounds were synthesized, characterized, and tested for their in vitro anti-stress, anti-obesity, and antimitotic properties. Thin layer chromatography method was used to verify the produced compound’s purity. IR, 13C and 1H NMR spectrum analyses were used to describe the produced substance. Stress is a feedback survival response that improves a person’s physical and mental health. An organism’s energy needs rise under stressful conditions, which leads to an increase in the production of free radicals. Oxidative stress is caused by the production of these free radicals. In the biomedical domain, malondialdehyde (MDA) is frequently employed as a biomarker for evaluating oxidative stress. In both in vitro and in vivo investigations, biomonitoring of MDA has been employed as a crucial biomarker for a number of disease patterns, including cancer, diabetes, atherosclerosis, heart failure and hypertension. The primary lipolytic enzyme produced and secreted by the pancreas, pancreatic lipase or triglycerolacyl hydrolase, is crucial for the effective digestion of triglycerides. Between 50 and 70 percent of all dietary lipids are hydrolyzed by pancreatic lipase. Long chain saturated and polyunsaturated fatty acids and β-monoglycerides are the lipolytic products that result from the removal of fatty acids from the α- addition of dietary triglycerides. An amazing possibility is presented by the bioactive heterocyclic molecules. One of the most extensively researched methods for assessing the possible effectiveness of anti-obesity medications is the inhibition of digestive enzymes. Chemical interactions in the human body can and do include most pharmaceutical drugs that imitate heterocyclic molecules. Allium cepa root meristematic cells, which have been widely employed in the screening of medications with antimitotic activity were used to screen antimitotic activity. The growth and mitotic activity of Allium cepa root meristems were assessed in order to compare the inhibitory effect of the produced compounds with that of the common anticancer medication methotrexate.
Graphical abstract