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<p><i>Mycoplasma pneumoniae</i> (MP) infection is known to trigger excessive activation of the NLRP3 inflammasome, contributing to inflammation-mediated cellular injury. To explore strategies for sensing and modulating such inflammatory responses, we developed a proof-of-concept dual-modal nanoplatform, 1-HA-APDEMS@2@Ceftriaxone, capable of simultaneous drug loading and real-time signal monitoring. The system exhibited distinct electrochemical and fluorescence responses, including an increase in charge-transfer resistance from 1.9 to 18.8&#xa0;kΩ, the appearance of a redox peak at +0.60&#xa0;V (sensitivity: 38.13&#xa0;μA&#xa0;μM<sup>−1</sup>; limit of detection: 1.2&#xa0;nM), and a threefold enhancement in fluorescence intensity through fluorescence resonance energy transfer (FRET), with a linear detection range of 5&#xa0;nM–2&#xa0;μM (<i>R</i><sup>2</sup> = 0.9969). In MP-infected A549 cells, the pH-responsive nanoplatform attenuated NLRP3 inflammasome activation and reduced IL-1β and IL-18 secretion, suggesting an immunomodulatory effect associated with nanoparticle-mediated drug delivery. Overall, this study provides preliminary evidence for a dual-functional nanosystem that integrates real-time monitoring of drug-carrier behavior with modulation of inflammation-related pathways. These findings establish a foundation for the future development of immunomodulatory nanomedicines for inflammatory diseases associated with MP infection.</p> Graphical abstract

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Porous hybrid material: Fluorescence-electrochemical behaviors and regulating NLRP3 inflammasome for Mycoplasma pneumonia treatment

  • 期刊论文

  • 发表日期：

    2026年7月17日

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  • Wenping Zhao,
  • Wei Zhang,
  • Jiping Li,
  • Leilei Qu

摘要

Mycoplasma pneumoniae (MP) infection is known to trigger excessive activation of the NLRP3 inflammasome, contributing to inflammation-mediated cellular injury. To explore strategies for sensing and modulating such inflammatory responses, we developed a proof-of-concept dual-modal nanoplatform, 1-HA-APDEMS@2@Ceftriaxone, capable of simultaneous drug loading and real-time signal monitoring. The system exhibited distinct electrochemical and fluorescence responses, including an increase in charge-transfer resistance from 1.9 to 18.8 kΩ, the appearance of a redox peak at +0.60 V (sensitivity: 38.13 μA μM−1; limit of detection: 1.2 nM), and a threefold enhancement in fluorescence intensity through fluorescence resonance energy transfer (FRET), with a linear detection range of 5 nM–2 μM (R2 = 0.9969). In MP-infected A549 cells, the pH-responsive nanoplatform attenuated NLRP3 inflammasome activation and reduced IL-1β and IL-18 secretion, suggesting an immunomodulatory effect associated with nanoparticle-mediated drug delivery. Overall, this study provides preliminary evidence for a dual-functional nanosystem that integrates real-time monitoring of drug-carrier behavior with modulation of inflammation-related pathways. These findings establish a foundation for the future development of immunomodulatory nanomedicines for inflammatory diseases associated with MP infection.

Graphical abstract