Scholarlink

<p>Cell lines following treatment with the extract at their respective IC₅₀ concentrations (35&#xa0;µg/ml for HT-29 and 20&#xa0;µg/ml for KB). Gene expression analysis showed upregulation of pro- Background: Natural products are a rich source of anticancer agents. <i>Tetraselmis suecica</i>, a green microalga, has demonstrated potential anticancer effects in limited studies, though its impact on colorectal and oral cancers remains unexplored. This study aims to investigate the anticancer and pro-apoptotic effects of <i>Tetraselmis suecica</i> extract on human colorectal adenocarcinoma cells (HT-29) and oral epidermal carcinoma cells (KB), and to evaluate its safety on normal human umbilical vein endothelial cells (HUVEC). The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to assess cell viability in HT-29, KB, and HUVEC cells. Apoptosis was evaluated using Annexin V/propidium iodide (PI) staining and flow cytometry. Real-time polymerase chain reaction (PCR) was performed to analyze the expression of apoptosis-related genes including <i>Caspase-3, Caspase-8, Caspase-9, Bax, Bcl-2, PTEN</i>, and <i>AKT</i>. <i>Tetraselmis suecica</i> extract reduced cell viability in HT-29 and KB cells in a dose-dependent manner, with IC₅₀ values of approximately 35&#xa0;µg/ml for HT-29 and 20&#xa0;µg/ml for KB cells, as calculated from dose–response curves. The extract showed minimal cytotoxicity on HUVEC cells, maintaining over 95% viability even at the highest tested concentration. Apoptosis was significantly increased in both cancer apoptotic genes (Caspases, Bax, PTEN) and downregulation of anti-apoptotic genes (Bcl-2, AKT). This study is the first to report the pro-apoptotic and anticancer effects of <i>Tetraselmis suecica</i> extract on oral and colorectal cancer cells. The findings suggest its potential as a natural therapeutic agent, with minimal cytotoxicity on normal endothelial cells.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Potential anticancer properties of Tetraselmis suecica extract against oral and colorectal cancer cells

  • 期刊论文

  • 发表日期：

    2025年6月25日

查看全文
  • Abbas Asoudeh-Fard,
  • Fatemeh Soltanmohammadi,
  • Maryam Kashkouie jahromi,
  • Fatemeh Javanmardi,
  • Anis Roosta,
  • Mohammad Zareian Jahromi,
  • Asghar Parsaei

摘要

Cell lines following treatment with the extract at their respective IC₅₀ concentrations (35 µg/ml for HT-29 and 20 µg/ml for KB). Gene expression analysis showed upregulation of pro- Background: Natural products are a rich source of anticancer agents. Tetraselmis suecica, a green microalga, has demonstrated potential anticancer effects in limited studies, though its impact on colorectal and oral cancers remains unexplored. This study aims to investigate the anticancer and pro-apoptotic effects of Tetraselmis suecica extract on human colorectal adenocarcinoma cells (HT-29) and oral epidermal carcinoma cells (KB), and to evaluate its safety on normal human umbilical vein endothelial cells (HUVEC). The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to assess cell viability in HT-29, KB, and HUVEC cells. Apoptosis was evaluated using Annexin V/propidium iodide (PI) staining and flow cytometry. Real-time polymerase chain reaction (PCR) was performed to analyze the expression of apoptosis-related genes including Caspase-3, Caspase-8, Caspase-9, Bax, Bcl-2, PTEN, and AKT. Tetraselmis suecica extract reduced cell viability in HT-29 and KB cells in a dose-dependent manner, with IC₅₀ values of approximately 35 µg/ml for HT-29 and 20 µg/ml for KB cells, as calculated from dose–response curves. The extract showed minimal cytotoxicity on HUVEC cells, maintaining over 95% viability even at the highest tested concentration. Apoptosis was significantly increased in both cancer apoptotic genes (Caspases, Bax, PTEN) and downregulation of anti-apoptotic genes (Bcl-2, AKT). This study is the first to report the pro-apoptotic and anticancer effects of Tetraselmis suecica extract on oral and colorectal cancer cells. The findings suggest its potential as a natural therapeutic agent, with minimal cytotoxicity on normal endothelial cells.