Scholarlink

<p>Whole glucan particles (WGP) enhance macrophage immune function, while serine protease inhibitor B9 (SerpinB9)—a molecule known to promote tumor immune escape—has an unclear role in WGP-induced macrophage function. In this study, we found that SerpinB9 knockout in WGP-induced bone marrow-derived macrophages (BMDMs) significantly downregulated surface MHC-II expression, reduced TNF-α secretion, and impaired their ability to induce Th1 cell differentiation. Transcriptome analysis revealed that CIITA, the core regulator of MHC-II transcription, was markedly downregulated in SerpinB9-knockout BMDMs. Further experiments confirmed that CIITA knockdown decreased BMDM surface MHC-II expression, whereas CIITA overexpression rescued MHC-II expression in SerpinB9-knockout BMDMs and restored their capacity to induce Th1 cell differentiation. In conclusion, this study demonstrates that under WGP induction, SerpinB9 regulates macrophage MHC-II expression and Th1 differentiation-inducing ability by maintaining CIITA expression, providing new insights into macrophage immune regulation mechanisms.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

SerpinB9 sustains CIITA to orchestrate MHC-II expression and Th1 differentiation in β-glucan-induced macrophages

  • 期刊论文

  • 发表日期：

    2025年10月29日

查看全文
  • Xin Huang,
  • Jun Ding,
  • Yongzhe Hao,
  • Jie Pan,
  • Meng Yuan,
  • Fang Shao,
  • Yewen Xie,
  • Zhichao Zhu,
  • Chunjian Qi

摘要

Whole glucan particles (WGP) enhance macrophage immune function, while serine protease inhibitor B9 (SerpinB9)—a molecule known to promote tumor immune escape—has an unclear role in WGP-induced macrophage function. In this study, we found that SerpinB9 knockout in WGP-induced bone marrow-derived macrophages (BMDMs) significantly downregulated surface MHC-II expression, reduced TNF-α secretion, and impaired their ability to induce Th1 cell differentiation. Transcriptome analysis revealed that CIITA, the core regulator of MHC-II transcription, was markedly downregulated in SerpinB9-knockout BMDMs. Further experiments confirmed that CIITA knockdown decreased BMDM surface MHC-II expression, whereas CIITA overexpression rescued MHC-II expression in SerpinB9-knockout BMDMs and restored their capacity to induce Th1 cell differentiation. In conclusion, this study demonstrates that under WGP induction, SerpinB9 regulates macrophage MHC-II expression and Th1 differentiation-inducing ability by maintaining CIITA expression, providing new insights into macrophage immune regulation mechanisms.