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<p>Autologous adipose tissue is a readily available source of mesenchymal stromal cells (MSCs) for regenerative applications. Here, human lipoaspirates from eight donors processed with the Lipogems system were characterized by flow cytometry (MSC/pericyte markers and inflammatory markers), immunocytochemistry (NANOG, OCT3/4, SOX2), and functional assays assessing proliferation, senescence, apoptosis, multilineage differentiation, IDO activity, and ROS levels. Lipogems-derived cultures fulfilled MSC immunophenotypic criteria while showing marked inter-donor variability in MSC/pericyte proportions, stemness-marker expression, differentiation bias, inflammatory profile, and oxidative status. In contrast, senescence and apoptosis were comparable across samples. Clinical evaluation, based on surgeon-assessed outcomes after autologous treatment, scored on a 1–10 scale, was consistently high across donors (7–10). Overall, Lipogems-processed lipoaspirates harbor a heterogeneous but functionally competent stromal compartment; these data support a sample-specific quality framework (composition, differentiation tendency, inflammation/ROS, IDO responsiveness) to improve reproducibility and optimization of regenerative protocols.</p>

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Molecular and Cellular Analysis of Lipogems-Processed Lipoaspirates for Evaluating the Efficacy of Treatments in Regenerative Medicine

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月11日

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  • Sura H. A. Al Sammarraie,
  • Nicola Alessio,
  • Domenico Aprile,
  • Beatrice Castiglioni,
  • Tiziana Squillaro,
  • Michela Bosetti,
  • Giovanni Di Bernardo

摘要

Autologous adipose tissue is a readily available source of mesenchymal stromal cells (MSCs) for regenerative applications. Here, human lipoaspirates from eight donors processed with the Lipogems system were characterized by flow cytometry (MSC/pericyte markers and inflammatory markers), immunocytochemistry (NANOG, OCT3/4, SOX2), and functional assays assessing proliferation, senescence, apoptosis, multilineage differentiation, IDO activity, and ROS levels. Lipogems-derived cultures fulfilled MSC immunophenotypic criteria while showing marked inter-donor variability in MSC/pericyte proportions, stemness-marker expression, differentiation bias, inflammatory profile, and oxidative status. In contrast, senescence and apoptosis were comparable across samples. Clinical evaluation, based on surgeon-assessed outcomes after autologous treatment, scored on a 1–10 scale, was consistently high across donors (7–10). Overall, Lipogems-processed lipoaspirates harbor a heterogeneous but functionally competent stromal compartment; these data support a sample-specific quality framework (composition, differentiation tendency, inflammation/ROS, IDO responsiveness) to improve reproducibility and optimization of regenerative protocols.