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<p>Aging is a multifaceted biological process that leads to structural and functional damage in the skin, mainly resulting from oxidative stress and inflammation. This study evaluates the protective effects of beta-caryophyllene (BCP) and vitamin D against oxidative stress-induced damage in human dermal fibroblasts (HDFs). Cell viability was assessed using the MTT assay after hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) induction, whereas reactive oxygen species (ROS) levels were measured by DCFH-DA staining. The expression of proteins and genes associated with inflammation and oxidative stress was analyzed by Western blotting and real-time RT-qPCR, respectively. The results demonstrated that both BCP and vitamin D were non-toxic to HDFs and effectively reduced ROS production by upregulating nuclear factor erythroid 2–related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) in H<sub>2</sub>O<sub>2</sub>-stimulated cells. Additionally, BCP treatment increased the expression of skin barrier-related genes, including fibrillin-1, collagen types I and III, and elastin, while suppressing inflammatory gene expression such as IL-6 and tumor necrosis factor-alpha (TNF-α). In addition, BCP significantly inhibited activation of the NF-κB, mTOR, Akt, ERK, and p38 signaling pathways, suggesting a protective role against oxidative stress. These findings suggest that BCP and vitamin D may serve as therapeutic agents for managing skin aging by reducing oxidative stress and inflammation. Future studies should explore their potential in dermatological therapies aimed at promoting skin health and resilience to age-related changes, using additional cell and animal models.</p>

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Beta-Caryophyllene Mitigates Oxidative Stress in Skin Fibroblasts by Targeting mTOR, Akt, and MAPK Signaling

  • 期刊论文

  • 发表日期：

    2025年10月29日

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  • Chanachinat Booraphet,
  • Wanwipha Woonnoi,
  • Wiwit Suttithumsatid,
  • Supita Tanasawet,
  • Nobuhiro Zaima,
  • Wanida Sukketsiri

摘要

Aging is a multifaceted biological process that leads to structural and functional damage in the skin, mainly resulting from oxidative stress and inflammation. This study evaluates the protective effects of beta-caryophyllene (BCP) and vitamin D against oxidative stress-induced damage in human dermal fibroblasts (HDFs). Cell viability was assessed using the MTT assay after hydrogen peroxide (H2O2) induction, whereas reactive oxygen species (ROS) levels were measured by DCFH-DA staining. The expression of proteins and genes associated with inflammation and oxidative stress was analyzed by Western blotting and real-time RT-qPCR, respectively. The results demonstrated that both BCP and vitamin D were non-toxic to HDFs and effectively reduced ROS production by upregulating nuclear factor erythroid 2–related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) in H2O2-stimulated cells. Additionally, BCP treatment increased the expression of skin barrier-related genes, including fibrillin-1, collagen types I and III, and elastin, while suppressing inflammatory gene expression such as IL-6 and tumor necrosis factor-alpha (TNF-α). In addition, BCP significantly inhibited activation of the NF-κB, mTOR, Akt, ERK, and p38 signaling pathways, suggesting a protective role against oxidative stress. These findings suggest that BCP and vitamin D may serve as therapeutic agents for managing skin aging by reducing oxidative stress and inflammation. Future studies should explore their potential in dermatological therapies aimed at promoting skin health and resilience to age-related changes, using additional cell and animal models.