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<p>To study the preliminary effect of the supercritical fluid extract of <i>Acacia auriculiformis</i> (<i>SEAA</i>) against breast cancer. Bark samples of <i>A. auriculiformis</i> were subjected to supercritical-fluid extraction, and the resulting <i>SEAA</i> was analyzed using gas chromatography-mass spectroscopy (GC-MS). Molecular docking was conducted to assess the interaction profile of betulin and doxorubicin with P21-activated kinase 4 (PAK-4), β-cell lymphoma 2 (BCL-2), and Schlafen-12 (SLFN-12). The cytotoxic and anti-proliferative effects of <i>SEAA</i> were examined through the brine shrimp lethality assay and <i>Allium cepa</i> assay. Three doses of <i>SEAA</i> (100&#xa0;µg/mL, 200&#xa0;µg/mL, and 300&#xa0;µg/mL), methotrexate (100&#xa0;µg/mL) as a standard group, and 0.7% saline as a normal control (NC) group were selected for both assays. The effect of <i>SEAA</i> on cell viability in tumor cells was evaluated through MTT assay on MCF-7 and HT-29 cell lines. GC-MS analysis confirmed the presence of betulin at a retention time of 5.662&#xa0;min. Betulin and doxorubicin demonstrated similar interaction profiles with PAK-4, BCL-2 and SLFN-12. <i>SEAA</i> treatment significantly (<i>P</i>-value &lt; 0.05) reduced nauplii survival and increased relative lethality. <i>SEAA</i> (100&#xa0;µg/mL) and <i>SEAA</i> (200&#xa0;µg/mL) treatments significantly (<i>P</i>-value &lt; 0.05) reduced the number and length of roots, while <i>SEAA</i> (300&#xa0;µg/mL) treatment produced the maximum reduction in the number (<i>P</i>-value &lt; 0.01) and length (<i>P</i>-value &lt; 0.05) of roots comparable with methotrexate (100&#xa0;µg/mL) treatment. <i>SEAA</i> demonstrated a dose-dependent reduction of cell viability in MCF-7 and HT-29 cell lines. <i>SEAA</i> treatment demonstrated preliminary cancer-protective potential through <i>in</i>-<i>silico</i> and <i>in</i>-<i>vitro</i> assays.</p>

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In-vitro and In-silico Evaluation of Supercritical Extract of Acacia Auriculiformis against Breast Cancer

  • 期刊论文

  • 发表日期：

    2025年9月15日

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  • Ruchi Dobariya,
  • Vaibhavi Savalia,
  • Pravin Tirgar,
  • Keval Raval

摘要

To study the preliminary effect of the supercritical fluid extract of Acacia auriculiformis (SEAA) against breast cancer. Bark samples of A. auriculiformis were subjected to supercritical-fluid extraction, and the resulting SEAA was analyzed using gas chromatography-mass spectroscopy (GC-MS). Molecular docking was conducted to assess the interaction profile of betulin and doxorubicin with P21-activated kinase 4 (PAK-4), β-cell lymphoma 2 (BCL-2), and Schlafen-12 (SLFN-12). The cytotoxic and anti-proliferative effects of SEAA were examined through the brine shrimp lethality assay and Allium cepa assay. Three doses of SEAA (100 µg/mL, 200 µg/mL, and 300 µg/mL), methotrexate (100 µg/mL) as a standard group, and 0.7% saline as a normal control (NC) group were selected for both assays. The effect of SEAA on cell viability in tumor cells was evaluated through MTT assay on MCF-7 and HT-29 cell lines. GC-MS analysis confirmed the presence of betulin at a retention time of 5.662 min. Betulin and doxorubicin demonstrated similar interaction profiles with PAK-4, BCL-2 and SLFN-12. SEAA treatment significantly (P-value < 0.05) reduced nauplii survival and increased relative lethality. SEAA (100 µg/mL) and SEAA (200 µg/mL) treatments significantly (P-value < 0.05) reduced the number and length of roots, while SEAA (300 µg/mL) treatment produced the maximum reduction in the number (P-value < 0.01) and length (P-value < 0.05) of roots comparable with methotrexate (100 µg/mL) treatment. SEAA demonstrated a dose-dependent reduction of cell viability in MCF-7 and HT-29 cell lines. SEAA treatment demonstrated preliminary cancer-protective potential through in-silico and in-vitro assays.