<p>Silver nanoparticles are widely used in many applications, such as water purification, dye degradation, drug delivery, and medicinal areas, to reduce inflammation and as an antioxidant agent. Therefore, the investigation is focused on an eco-friendly way for the synthesis of silver nanoparticles from <i>Macrotyloma uniflorum</i> seeds starch and its applications in different areas, such as anti-inflammatory, antioxidant, catalytic activity, and DPPH-scavenging activity. As seen in the in vitro results, the catalytic activity of AgNPs was significant, NPs were immobilized in starch-chitosan beads and used in Congo red dye degradation. The peak position at 414 indicates the signature of silver nanoparticles, which is investigated by UV- spectroscopy. Characterization of AgNPs was carried out using TEM, DLS, and FTIR spectroscopy. Particle size is in the range of 7–10&#xa0;nm, as analyzed by using TEM and 220&#xa0;nm by DLS. Anti-inflammatory, H₂O₂, and DPPH activities of silver nanoparticles are significant, and the percentage of inhibition is seen to be the same as the control. Catalytic activity observed in the degradation of Congo red dye in 10&#xa0;min by using immobilized silver nanoparticles in starch-chitosan beads, and recycled a minimum of 25 times. All the above-mentioned applications of AgNPs are very useful in the medicinal and industrial areas.</p> Graphical Abstract <p></p>