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<p>Laccase from <i>Trametes versicolor</i> was immobilized on a Cu-silica aerogel carrier with a large surface area (370&#xa0;m²/g). Its catalytic efficacy against various synthetic dyes was thoroughly evaluated. Under optimal immobilization conditions (25&#xa0;°C, pH 4.5, 2% glutaraldehyde, 12&#xa0;mg/g enzyme loading, 4&#xa0;h), immobilization efficiency and activity retention reached 93%, demonstrating the effectiveness of the immobilization strategy. After 10 reuse cycles, the immobilized enzyme retained 80% of its original activity, indicating high operational durability. Additionally, the immobilized biocatalyst exhibited greater stability across a broader temperature range (up to 60&#xa0;°C) and at pH 4.5 compared to the free enzyme. The kinetic data for 2,6-dimethoxyphenol (2,6-DMP) showed a K<sub>m</sub> of 0.20 mM (free enzyme: 0.10 mM) and a V<sub>max</sub> of 178 µmol min⁻¹ mL⁻¹ (free enzyme: 69 µmol min⁻¹ mL⁻¹). For ABTS, the immobilized system had a K<sub>m</sub> of 0.35 mM (free: 0.20 mM) and a V<sub>max</sub> of 278 µmol min⁻¹ mL⁻¹ (free: 500 µmol min⁻¹ mL⁻¹), indicating a change in substrate affinity after immobilization. The enhanced catalytic efficiency of the immobilized enzyme was further confirmed by dye degradation tests, which showed 95% elimination of Methylene Blue within 24&#xa0;h and complete removal of Coomassie Brilliant Blue G-250 and Congo Red. This performance significantly exceeded that of the free enzyme. Therefore, Cu-silica aerogel-immobilized laccase is a strategically valuable biocatalyst with high catalytic efficiency, strong operational stability, and promising scalability, offering significant potential for industrial wastewater treatment and advanced environmental biotechnology applications.</p>

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Immobilization of Trametes versicolor Laccase Enzyme to Cu-silica Aerogel and its Decolorization of Synthetic Dyes

  • 期刊论文

  • 发表日期：

    2026年2月28日

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  • Semih Isik,
  • Sevil Yucel,
  • Yonca Yuzugullu Karakus

摘要

Laccase from Trametes versicolor was immobilized on a Cu-silica aerogel carrier with a large surface area (370 m²/g). Its catalytic efficacy against various synthetic dyes was thoroughly evaluated. Under optimal immobilization conditions (25 °C, pH 4.5, 2% glutaraldehyde, 12 mg/g enzyme loading, 4 h), immobilization efficiency and activity retention reached 93%, demonstrating the effectiveness of the immobilization strategy. After 10 reuse cycles, the immobilized enzyme retained 80% of its original activity, indicating high operational durability. Additionally, the immobilized biocatalyst exhibited greater stability across a broader temperature range (up to 60 °C) and at pH 4.5 compared to the free enzyme. The kinetic data for 2,6-dimethoxyphenol (2,6-DMP) showed a Km of 0.20 mM (free enzyme: 0.10 mM) and a Vmax of 178 µmol min⁻¹ mL⁻¹ (free enzyme: 69 µmol min⁻¹ mL⁻¹). For ABTS, the immobilized system had a Km of 0.35 mM (free: 0.20 mM) and a Vmax of 278 µmol min⁻¹ mL⁻¹ (free: 500 µmol min⁻¹ mL⁻¹), indicating a change in substrate affinity after immobilization. The enhanced catalytic efficiency of the immobilized enzyme was further confirmed by dye degradation tests, which showed 95% elimination of Methylene Blue within 24 h and complete removal of Coomassie Brilliant Blue G-250 and Congo Red. This performance significantly exceeded that of the free enzyme. Therefore, Cu-silica aerogel-immobilized laccase is a strategically valuable biocatalyst with high catalytic efficiency, strong operational stability, and promising scalability, offering significant potential for industrial wastewater treatment and advanced environmental biotechnology applications.