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<p>The ultrasonic-assisted deep eutectic solvents (DES) method was used to extract the polysaccharides from Abri Herba(AHPs), and the extraction process was optimized by Box-Behnken response surface. The optimal conditions were determined as follows: the molar ratio of choline chloride and urea of 1:3, water content of DES of 70%, ultrasonic temperature of 61&#xa0;°C, ultrasonic time of 58&#xa0;min and the ratio of liquid to solid of 50 mL/g. Under these conditions, the extraction yield of AHPs reached 8.49 ± 0.34%. After purification of AHPs using a DEAE-52 cellulose column, the fraction designated as AHPs-1 displayed a single symmetrical peak. The molecular weight (<i>M</i><sub><i>W</i></sub>) of AHPs-1 was determined to be 4.74 × 10<sup>4</sup> Da by HPSEC-MALLS-RID chromatography system, with a narrow polydispersity index of 1.89, confirming its homogeneous nature. FT-IR spectra demonstrated typical characteristic peaks of polysaccharides, while Congo red assay ruled out a triple-helix structure. The scanning electron microscope (SEM) showed that AHPs-1 exhibited a rough surface morphology with irregular granular protuberances. Hypoglycemic activity assays demonstrated significant inhibitory effects of AHPs-1 on both α-glucosidase and α-amylase. Therefore, this study establishes an efficient DES-based extraction method for AHPs and identifies AHPs-1 as a potential hypoglycemic agent for functional foods and pharmaceuticals.</p>

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Optimization of Ultrasound-Assisted Deep Eutectic Solvents Extraction, Characterization, and Hypoglycemic Activity of Polysaccharides from Abri Herba

  • 期刊论文

  • 发表日期：

    2026年1月13日

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  • Tao Yang,
  • Fengjuan Yang,
  • Danni Xu,
  • Guangjin Zheng,
  • Cuizhong Zhang,
  • Wenxi Wang

摘要

The ultrasonic-assisted deep eutectic solvents (DES) method was used to extract the polysaccharides from Abri Herba(AHPs), and the extraction process was optimized by Box-Behnken response surface. The optimal conditions were determined as follows: the molar ratio of choline chloride and urea of 1:3, water content of DES of 70%, ultrasonic temperature of 61 °C, ultrasonic time of 58 min and the ratio of liquid to solid of 50 mL/g. Under these conditions, the extraction yield of AHPs reached 8.49 ± 0.34%. After purification of AHPs using a DEAE-52 cellulose column, the fraction designated as AHPs-1 displayed a single symmetrical peak. The molecular weight (MW) of AHPs-1 was determined to be 4.74 × 104 Da by HPSEC-MALLS-RID chromatography system, with a narrow polydispersity index of 1.89, confirming its homogeneous nature. FT-IR spectra demonstrated typical characteristic peaks of polysaccharides, while Congo red assay ruled out a triple-helix structure. The scanning electron microscope (SEM) showed that AHPs-1 exhibited a rough surface morphology with irregular granular protuberances. Hypoglycemic activity assays demonstrated significant inhibitory effects of AHPs-1 on both α-glucosidase and α-amylase. Therefore, this study establishes an efficient DES-based extraction method for AHPs and identifies AHPs-1 as a potential hypoglycemic agent for functional foods and pharmaceuticals.