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<p>Levansucrase Ba-SacB from <i>Bacillus amyloliquefaciens</i> catalyses the production of levan using sucrose as substrate, and levan is widely used in food industry and medicine. Here the support of nano zinc oxide is used for efficient immobilization of Ba-SacB (i.e., Ba-SacB-ZnO). FT-IR analysis revealed that Ba-SacB got absorbed via electrostatic interaction with the support. The Ba-SacB-ZnO showed improved activity in the pH range 3–5, and improved pH stability in the pH range 6–9. The Ba-SacB-ZnO kept nearly 70% activity after 21 d of storage, which was 20% higher than that of its free counterpart. In addition, the Ba-SacB-ZnO showed better tolerance to multiple chemicals, metal ions, organic solvents, proteases than the free Ba-SacB, suggesting that Ba-SacB-ZnO could be efficient for applications requiring in more diverse, especially harsh environments. About 50% of Ba-SacB-ZnO retained activity after 10 cycles of reuse. This study provides a new idea for efficient production of levan.</p>

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Immobilization of Levansucrase Ba-SacB from Bacillus Amyloliquefaciens on Nano Zinc Oxide

  • 期刊论文

  • 发表日期：

    2026年1月10日

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  • Song Gao,
  • Mingxiong Tan,
  • Ran Lu,
  • Guoshi Kan,
  • Jayaweera Arachchige Asela Sampath Jayaweera,
  • Yingfeng An

摘要

Levansucrase Ba-SacB from Bacillus amyloliquefaciens catalyses the production of levan using sucrose as substrate, and levan is widely used in food industry and medicine. Here the support of nano zinc oxide is used for efficient immobilization of Ba-SacB (i.e., Ba-SacB-ZnO). FT-IR analysis revealed that Ba-SacB got absorbed via electrostatic interaction with the support. The Ba-SacB-ZnO showed improved activity in the pH range 3–5, and improved pH stability in the pH range 6–9. The Ba-SacB-ZnO kept nearly 70% activity after 21 d of storage, which was 20% higher than that of its free counterpart. In addition, the Ba-SacB-ZnO showed better tolerance to multiple chemicals, metal ions, organic solvents, proteases than the free Ba-SacB, suggesting that Ba-SacB-ZnO could be efficient for applications requiring in more diverse, especially harsh environments. About 50% of Ba-SacB-ZnO retained activity after 10 cycles of reuse. This study provides a new idea for efficient production of levan.