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<p>Carotenoids act as the precursors of vitamin A and natural antioxidants, which benefit human health. The APETALA2/Ethylene Responsive Factor (AP2/ERF), as a transcription factor family, involved in various biological processes of higher plants, such as playing a key role in regulation of effective carotenoid accumulation. Several reports showed that plant′s AP2/ERF transcription factors (TFs) could response to various stresses, plant morphogenesis, hormone signal transduction and metabolite regulation. Nevertheless, the understanding of the regulatory mechanism of AP2/ERF TF in marine microalga <i>Dunaliella parva</i> (<i>D. parva</i>) has been much more limited, which remains to be elucidated. Here, <i>DpAP2</i> gene belonging to <i>AP2/ERF</i> family was identified in <i>D. parva</i>. To reveal the function of DpAP2, the targets of DpAP2 were identified by chromatin immunoprecipitation (ChIP) sequencing. The results showed that 902 DpAP2-binding genes were identified by ChIP sequencing (ChIP-seq) analyses. Gene enrichment analysis indicated that these binding genes were related to RNA transport, carbon metabolism, biosynthesis of secondary metabolites, fatty acid metabolism and other functions. Most of these identified genes were reported to involve in carotenoid biosynthesis. In addition, DpAP2-regulated target genes participated in carbohydrate metabolism (including starch and sucrose metabolism, photosynthesis, glycolysis/gluconeogenesis, and tricarboxylic acid cycle) to improve the production of carotenoid. DpAP2<i>-</i>regulated target genes related to carotenoid biosynthesis (such as <i>Z-ISO</i>, <i>CRTISO</i> and <i>AOG</i>) to promote the content of carotenoids. The studies of <i>DpAP2</i> in <i>D. parva</i> will provide an important foundation for a deep understanding of the regulatory mechanism and microalgal breeding.</p>

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Identification of Target Genes of Transcription Factor DpAP2 Related to Carotenoid Biosynthesis from Dunaliella Parva

  • 期刊论文

  • 发表日期：

    2026年1月12日

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  • Jin Zhang,
  • Lei Mo,
  • Jiaming Jiang,
  • Yu Huo,
  • Yuling Bao,
  • Bingbing Pang,
  • Changhua Shang

摘要

Carotenoids act as the precursors of vitamin A and natural antioxidants, which benefit human health. The APETALA2/Ethylene Responsive Factor (AP2/ERF), as a transcription factor family, involved in various biological processes of higher plants, such as playing a key role in regulation of effective carotenoid accumulation. Several reports showed that plant′s AP2/ERF transcription factors (TFs) could response to various stresses, plant morphogenesis, hormone signal transduction and metabolite regulation. Nevertheless, the understanding of the regulatory mechanism of AP2/ERF TF in marine microalga Dunaliella parva (D. parva) has been much more limited, which remains to be elucidated. Here, DpAP2 gene belonging to AP2/ERF family was identified in D. parva. To reveal the function of DpAP2, the targets of DpAP2 were identified by chromatin immunoprecipitation (ChIP) sequencing. The results showed that 902 DpAP2-binding genes were identified by ChIP sequencing (ChIP-seq) analyses. Gene enrichment analysis indicated that these binding genes were related to RNA transport, carbon metabolism, biosynthesis of secondary metabolites, fatty acid metabolism and other functions. Most of these identified genes were reported to involve in carotenoid biosynthesis. In addition, DpAP2-regulated target genes participated in carbohydrate metabolism (including starch and sucrose metabolism, photosynthesis, glycolysis/gluconeogenesis, and tricarboxylic acid cycle) to improve the production of carotenoid. DpAP2-regulated target genes related to carotenoid biosynthesis (such as Z-ISO, CRTISO and AOG) to promote the content of carotenoids. The studies of DpAP2 in D. parva will provide an important foundation for a deep understanding of the regulatory mechanism and microalgal breeding.