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<p>Propagation in in vitro conditions offers significant advantages such as faster, mass production and disease, virus free plant production. In in vitro culture, the addition of auxin and cytokinin plant growth regulators to the nutrient medium is very efficient for cell differentiation, shoot multiplication, organ formation and tillering. In this context, it was aimed to determine the effects of different plant growth regulators (BAP and NAA) combinations added to MS nutrient medium on shoot regeneration and development of shoots in in vitro culture of <i>Aronia melanocarpa</i> (Michx) Elliott. Rootless shoot explants were cultured in prepared MS nutrient media containing different concentrations of BAP (0.6, 0.7, 1.0, 2.0, 3.0 and 4.0 mg.L<sup>− 1</sup>) and NAA (0.05, 0.01, 0.5, 0.1 and 1.0 mg.L<sup>− 1</sup>) for 8 weeks. At the end of culture, the regeneration situation in the cultured explants and the morphological characteristics of the developing shoots were examined. Within the scope of morphological characteristics; the highest length of shoot (cm), average shoot length (cm), number of shoots per explant (tillering) (number), longest root length (cm), number of leaves per shoot (unit), chlorophyll density, rooted explant rate (%) and callus forming explant rate (%) parameters were taken into account in the measurements. As a result of the research; the highest values were determined for the average shoot length in MS medium containing 3 mg.L<sup>− 1</sup> BAP + 0.5 mg.L<sup>− 1</sup> NAA (M3) as 2.52&#xa0;cm; the number of shoots per explant in MS medium containing 2 mg.L<sup>− 1</sup> BAP (M10) and in MS medium containing 3 mg.L<sup>− 1</sup> BAP + 0.1 mg.L<sup>− 1</sup> NAA (M7). And also M8 medium containing 0.7 mg.L<sup>− 1</sup> BAP + 0.05 mg.L<sup>− 1</sup> NAA gave the best result in terms of longest root length. However, the rate of explants forming callus, chlorophyll density, number of leaves per shoot and the highest shoot length were not found to be different with the doses of BAP and NAA added to the nutrient media. As a result; It was determined that the dose of BAP added to the MS nutrient medium contributed to shoot and organ formation and development, being higher than the dose of NAA.</p>

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Effects of Different Dose Combinations of 6-Benzyl Amino Purine and Naphthalene Acetic Acid on Shoot Regeneration and Some Morphological Characteristics of New Shoots in Micropropagation of Aronia melanocarpa (Michx) Elliott. ‘Viking’

  • 期刊论文

  • 发表日期：

    2025年11月8日

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  • Uğur Şirin,
  • Merve Kabakcı,
  • Cansu Di̇ndar

摘要

Propagation in in vitro conditions offers significant advantages such as faster, mass production and disease, virus free plant production. In in vitro culture, the addition of auxin and cytokinin plant growth regulators to the nutrient medium is very efficient for cell differentiation, shoot multiplication, organ formation and tillering. In this context, it was aimed to determine the effects of different plant growth regulators (BAP and NAA) combinations added to MS nutrient medium on shoot regeneration and development of shoots in in vitro culture of Aronia melanocarpa (Michx) Elliott. Rootless shoot explants were cultured in prepared MS nutrient media containing different concentrations of BAP (0.6, 0.7, 1.0, 2.0, 3.0 and 4.0 mg.L− 1) and NAA (0.05, 0.01, 0.5, 0.1 and 1.0 mg.L− 1) for 8 weeks. At the end of culture, the regeneration situation in the cultured explants and the morphological characteristics of the developing shoots were examined. Within the scope of morphological characteristics; the highest length of shoot (cm), average shoot length (cm), number of shoots per explant (tillering) (number), longest root length (cm), number of leaves per shoot (unit), chlorophyll density, rooted explant rate (%) and callus forming explant rate (%) parameters were taken into account in the measurements. As a result of the research; the highest values were determined for the average shoot length in MS medium containing 3 mg.L− 1 BAP + 0.5 mg.L− 1 NAA (M3) as 2.52 cm; the number of shoots per explant in MS medium containing 2 mg.L− 1 BAP (M10) and in MS medium containing 3 mg.L− 1 BAP + 0.1 mg.L− 1 NAA (M7). And also M8 medium containing 0.7 mg.L− 1 BAP + 0.05 mg.L− 1 NAA gave the best result in terms of longest root length. However, the rate of explants forming callus, chlorophyll density, number of leaves per shoot and the highest shoot length were not found to be different with the doses of BAP and NAA added to the nutrient media. As a result; It was determined that the dose of BAP added to the MS nutrient medium contributed to shoot and organ formation and development, being higher than the dose of NAA.