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<p>This study overexpressed <i>DpTF</i> in <i>D. parva</i> to investigate its effects on organic content and antioxidant activity. The transgenic <i>D. parva</i> with recombinant vector pBI221-UbiΩ-CAT-DpTF was <i>D. parva</i> T. The transgenic <i>D. parva</i> with empty vector pBI221-GFP-UbiΩ-CAT was <i>D. parva</i> C. Compared with <i>D. parva</i> C, protein contents of <i>D. parva</i> T decreased by 34.3 and 48.8 mg/g DW at 12 days and 15 days, respectively. Compared with <i>D. parva</i> C, carbohydrate contents of <i>D. parva</i> T decreased by 9 and 6.2 mg/g DW, respectively. Compared with <i>D. parva</i> C, the contents of chlorophyll a, chlorophyll b, and carotenoid decreased by 47.5%, 43.1%, and 43.27% in <i>D. parva</i> T, respectively. Compared with <i>D. parva</i> C, reducing powers of <i>D. parva</i> T decreased by 3.3% and 6.3%/mg at 12 days and 15 days, and hydroxyl radical scavenging activity of <i>D. parva</i> T decreased by 24.8%/mg at 12 days. Oil contents of <i>D. parva</i> T decreased by 3.5% and 14.7% at 12 days and 15 days, compared with <i>D. parva</i> C. It was inferred that <i>DpTF</i> overexpression resulted in the reduced mRNA levels of <i>DpWRI1-like</i> and <i>DpAP2</i>, further reducing oil content and carotenoid content. The results showed that <i>DpTF</i> had inhibitory effects on organic contents and antioxidant activities.</p> Graphical Abstract <p></p>

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Effects of DpTF Gene Overexpression on Dunaliella parva

  • 期刊论文

  • 发表日期：

    2025年10月9日

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  • Jin Zhang,
  • Qing Wang,
  • Lei Mo,
  • Limei Huang,
  • Lingru Ruan,
  • Shiyu Liang,
  • Changhua Shang

摘要

This study overexpressed DpTF in D. parva to investigate its effects on organic content and antioxidant activity. The transgenic D. parva with recombinant vector pBI221-UbiΩ-CAT-DpTF was D. parva T. The transgenic D. parva with empty vector pBI221-GFP-UbiΩ-CAT was D. parva C. Compared with D. parva C, protein contents of D. parva T decreased by 34.3 and 48.8 mg/g DW at 12 days and 15 days, respectively. Compared with D. parva C, carbohydrate contents of D. parva T decreased by 9 and 6.2 mg/g DW, respectively. Compared with D. parva C, the contents of chlorophyll a, chlorophyll b, and carotenoid decreased by 47.5%, 43.1%, and 43.27% in D. parva T, respectively. Compared with D. parva C, reducing powers of D. parva T decreased by 3.3% and 6.3%/mg at 12 days and 15 days, and hydroxyl radical scavenging activity of D. parva T decreased by 24.8%/mg at 12 days. Oil contents of D. parva T decreased by 3.5% and 14.7% at 12 days and 15 days, compared with D. parva C. It was inferred that DpTF overexpression resulted in the reduced mRNA levels of DpWRI1-like and DpAP2, further reducing oil content and carotenoid content. The results showed that DpTF had inhibitory effects on organic contents and antioxidant activities.

Graphical Abstract