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<p>The limited stability and insufficient recyclability of lipase (CRL), a crucial biocatalyst, hinder its extensive industrial application. In this study, we successfully immobilized CRL on a magnetic graphene surface by using polyethyleneimine and polydopamine (PEI/PDA) bionic adhesive as a flexible connecting branch and amino acid ionic liquid as an activator. The immobilized enzyme exhibited markedly enhanced thermal stability and denaturant tolerance. Specifically, the immobilized enzyme retained more than 80% activity after incubation at 60&#xa0;°C for 1&#xa0;h, which was approximately 1.8 times higher than that of the free enzyme. Additionally, following incubation in 6&#xa0;mol/L denaturant for 2&#xa0;h, the immobilized enzyme maintained over 80% activity. Moreover, the immobilized enzyme displayed superior storage stability and magnetic properties, significantly simplifying the recovery process. Notably, even after seven reuses, the immobilized enzyme retained more than 70% of its catalytic activity. When the immobilized enzyme was applied to the synthesis of phytosterol esters, an esterification rate of 90.2% was achieved. Moreover, the enzyme maintained a high activity level, with the esterification rate still exceeding 70% after five cycles of reuse, demonstrating excellent reusability. This research would not only offer innovative strategies for lipase immobilization but also establish a robust theoretical framework for the application of ionic liquids in immobilized enzyme technology, thereby providing important support for the industrial application of biocatalysts.</p>

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Amino Acid Ionic Liquid Activation of Biomimetic Magnetic Graphene for Enhancing Lipase Stability and Catalytic Performance

  • 期刊论文

  • 发表日期：

    2025年10月11日

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  • Junfeng Wang,
  • Yongjin Sheng,
  • Suomin Wang,
  • Can Li,
  • Kai Shen,
  • Zhenfang Liu,
  • Futong Liu,
  • Siyu Tian,
  • Yanmei Zhou,
  • Tianyi Huang,
  • Wei Zhang

摘要

The limited stability and insufficient recyclability of lipase (CRL), a crucial biocatalyst, hinder its extensive industrial application. In this study, we successfully immobilized CRL on a magnetic graphene surface by using polyethyleneimine and polydopamine (PEI/PDA) bionic adhesive as a flexible connecting branch and amino acid ionic liquid as an activator. The immobilized enzyme exhibited markedly enhanced thermal stability and denaturant tolerance. Specifically, the immobilized enzyme retained more than 80% activity after incubation at 60 °C for 1 h, which was approximately 1.8 times higher than that of the free enzyme. Additionally, following incubation in 6 mol/L denaturant for 2 h, the immobilized enzyme maintained over 80% activity. Moreover, the immobilized enzyme displayed superior storage stability and magnetic properties, significantly simplifying the recovery process. Notably, even after seven reuses, the immobilized enzyme retained more than 70% of its catalytic activity. When the immobilized enzyme was applied to the synthesis of phytosterol esters, an esterification rate of 90.2% was achieved. Moreover, the enzyme maintained a high activity level, with the esterification rate still exceeding 70% after five cycles of reuse, demonstrating excellent reusability. This research would not only offer innovative strategies for lipase immobilization but also establish a robust theoretical framework for the application of ionic liquids in immobilized enzyme technology, thereby providing important support for the industrial application of biocatalysts.