<p>Biofilm formation poses challenges across various sectors, such as healthcare facilities, food safety, and in industrial processes, owing to the resilience of microbial communities encased in protective extracellular matrices. This paper delves into the strategies for biofilm control, highlighting recent/novel chemical, biological, and nanotechnological approaches. Chemical methods exploit the potential of natural compounds, such as phenolic antioxidants and nanoparticles, to disrupt biofilms, thereby boosting the effectiveness of antimicrobial treatments. In parallel, biological strategies involve the use of predatory bacteria and biosurfactants and offer eco-friendly alternatives. In nanotechnology, particularly the nanoflowers, due to their unique morphology and high surface area, show greater promise in efficiently targeting biofilms by enabling enhanced bacterial interaction and precise drug delivery. The implications of these approaches are far-reaching, extending to the food industry, where biofilm formation can result in spoilage and foodborne illnesses, as well as in biomedical applications aimed at preventing infections related to medical devices. This paper underscores the potential of integrating these approaches to develop more effective and sustainable solutions for biofilm management, contributing to enhanced health and safety across multiple domains.</p>