<p>In the field of human organ printing, there are problems with low printing planning accuracy and difficult speed control. In this regard, the research proposes a trajectory planning and speed control technology with significant novelty: innovatively using the improved B-spline algorithm to optimize the path continuity planning, while introducing the seven segment S-shaped curve algorithm, and combining the two-way forward-looking strategy to achieve accurate speed control. In the trajectory path planning experiment, the coincidence rate of the breakpoint tracking link in the research technology is 94.5%, and there are no jumps, indicating the best overall performance. In error control, the research technology error is within the range of 0.015&#xa0;cm. In the speed control experiment, the control accuracy is highest when the research technology is within the range of ± 0.1&#xa0;mm/s in constant speed control. In the comparison of printing time and material consumption among different models, the average time and material consumption of the research technology are 91.5&#xa0;min and 9125&#xa0;mm, respectively, showing the best overall performance. The research technology can effectively improve the printing effect of human organs. This technology effectively improves the printing effect of human organs, and its innovative method provides a key reference for the technical development and application promotion in the field of bioprinting.</p> Graphical abstract <p></p>