<p>In a hemostatic ultrasonic scalpel, high-frequency ultrasonic vibrations generate localized heat, leading to simultaneous cutting and coagulation of the area in contact with the tissue. Optimizing the design parameters results in more precise cutting and better tissue coagulation. This research involves optimization of the design and evaluation of the performance of an ultrasonic surgical scalpel. The design was carried out using an electromechanical equivalent circuit method and modal and harmonic analysis in finite element software, along with optimization of geometric parameters using response surface methodology. The objective of optimization is to achieve a resonance frequency of 55.5 kHz in the longitudinal mode and to optimize the vibration amplitudes in the important areas of the ultrasonic scalpel. To evaluate the scalpel, impedance analyzing tests, displacement amplitude measurement of the horn tip, and experimentation on a sheep’s vessel have been conducted. Cutting and sealing the vessel using the ultrasonic scalpel demonstrates that the frequency and amplitude (40 μm) of the surgical tool are suitable for vessel cutting and sealing. A comparison of simulation results and experimental testing indicates an error of less than 4 percent in the simulations. Through modeling and optimization, it became possible to achieve an efficient ultrasonic scalpel and conduct a precise evaluation of its characteristics.</p>