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<p>The gut microbiota is essential to human health, with dietary polysaccharides serving as major regulators of its composition and metabolic activity. This study sought to improve the prebiotic properties of polysaccharides derived from <i>Brassica rapa</i> L. through structural modification and to quantitatively assess their effects on gut microbial composition and short-chain fatty acid (SCFAs) production. Native polysaccharides (BRL-L) were degraded using ultrasound-assisted hydrogen peroxide treatment, and their prebiotic potential was evaluated through continuous in vitro bioreactor fermentation designed to mimic the colonic environment. After 48&#xa0;h of fermentation, the degraded BRL-L underwent a pronounced structural change, from cross-linked aggregates to discrete fragments, along with a shift in molecular weight (<i>Mw</i>) distribution from a bimodal profile (200,479&#xa0;Da, 31.39%; 5,641&#xa0;Da, 68.61%) to a monodisperse form (1,983&#xa0;Da, 100%). The monosaccharide profile of BRL-L changed markedly, with fucose content decreasing from 6.26% to 2.93% and xylose content rising from 23.93% to 31.83%. Relative to the control (Con), BRL-L fermentation reduced total carbohydrate levels in the fermentate from 16.81&#xa0;mg/mL to 6.57&#xa0;mg/mL, while substantially increasing SCFAs production from 9.49&#xa0;mM to 59.30&#xa0;mM. Microbiota analysis showed pronounced structural modulation, with BRL-L lowering the relative abundance of Firmicutes and increasing Bacteroidota, resulting in a reduced <i>Firmicutes</i>/<i>Bacteroidota</i> ratio. Additionally, BRL-L degradation selectively promoted SCFAs-producing genera, notably increasing the relative abundance of <i>Lachnoclostridium</i> and <i>Faecalibacterium</i>. Overall, the degraded BRL-L exhibits strong potential as a functional prebiotic, capable of restoring microbial balance in the gut and markedly boosting the synthesis of health-promoting SCFAs.</p> Graphical Abstract <p>Thank you for the assistance provided by the home for researchers (<a href="http://www.home-for-researchers.com">www.home-for-researchers.com</a>)</p> <p></p>

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Effect of In Vitro Colonic Fermentation on the Physicochemical, Structural, and Prebiotic Properties of Ultrasound-H₂O₂ Degraded Brassica Rapa L. Polysaccharides

  • 期刊论文

  • 发表日期：

    2025年12月20日

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  • Dongdong Guo,
  • Jiayu Lei,
  • Wenlida Mu,
  • Jin Hua,
  • Jun Liu,
  • Chang Liu,
  • He Qian

摘要

The gut microbiota is essential to human health, with dietary polysaccharides serving as major regulators of its composition and metabolic activity. This study sought to improve the prebiotic properties of polysaccharides derived from Brassica rapa L. through structural modification and to quantitatively assess their effects on gut microbial composition and short-chain fatty acid (SCFAs) production. Native polysaccharides (BRL-L) were degraded using ultrasound-assisted hydrogen peroxide treatment, and their prebiotic potential was evaluated through continuous in vitro bioreactor fermentation designed to mimic the colonic environment. After 48 h of fermentation, the degraded BRL-L underwent a pronounced structural change, from cross-linked aggregates to discrete fragments, along with a shift in molecular weight (Mw) distribution from a bimodal profile (200,479 Da, 31.39%; 5,641 Da, 68.61%) to a monodisperse form (1,983 Da, 100%). The monosaccharide profile of BRL-L changed markedly, with fucose content decreasing from 6.26% to 2.93% and xylose content rising from 23.93% to 31.83%. Relative to the control (Con), BRL-L fermentation reduced total carbohydrate levels in the fermentate from 16.81 mg/mL to 6.57 mg/mL, while substantially increasing SCFAs production from 9.49 mM to 59.30 mM. Microbiota analysis showed pronounced structural modulation, with BRL-L lowering the relative abundance of Firmicutes and increasing Bacteroidota, resulting in a reduced Firmicutes/Bacteroidota ratio. Additionally, BRL-L degradation selectively promoted SCFAs-producing genera, notably increasing the relative abundance of Lachnoclostridium and Faecalibacterium. Overall, the degraded BRL-L exhibits strong potential as a functional prebiotic, capable of restoring microbial balance in the gut and markedly boosting the synthesis of health-promoting SCFAs.

Graphical Abstract

Thank you for the assistance provided by the home for researchers (www.home-for-researchers.com)