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<p>The significant health risks associated with ochratoxin A (OTA) contamination in multiple types of foodstuffs underscore the need for detection methods. The inherent complexity of the real sample matrix significantly prolongs OTA analysis time, and the intricate procedural steps present obstacles that must be overcome. Therefore, effective pretreatment is&#xa0;essential. In this study, aptamer-functionalized superparamagnetic chitosan nanoparticles (Apt-SPCO) were synthesized and utilized as adsorbents&#xa0;for the rapid purification and efficient detection&#xa0;of OTA in various real samples, Furthermore, the recognition and binding mechanism between Apt-SPCO and OTA was investigated. Apt-SPCO is spherical in shape, possessing superparamagnetic and anti-interference ability, which can effectively extract and purify OTA in 10&#xa0;min, and coupled with liquid chromatography (HPLC-FLD) detection method the recovery was 90.9% ~ 106.4% in real sample. Hydrogen bonds were formed between OTA and aptamer (DG-9 bases), with the <i>K</i><sub><i>d</i></sub> of 2.72&#xa0;μM, indicating a high affinity between OTA and aptamer. Meanwhile, Ca<sup>2+</sup> induced the aptamer structure changing from a parallel G-quadruplex to an anti-parallel G-quadruplex. These results underscore the user-friendliness, affordable, and excellent selectivity of Apt-SPCO pretreatment. When combined with HPLC-FLD, it exhibits high sensitivity and satisfactory accuracy for OTA determination, particularly in complex food matrix.</p>

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Mechanism and Determination of OTA in Complex Food Matrix Based on Rapid and Selective Extraction via Aptamer-Functionalized Superparamagnetic Chitosan Nanoparticles

  • 期刊论文

  • 发表日期：

    2025年12月16日

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  • Yingying Sheng,
  • Xueyan Xin,
  • Mina Nan,
  • Yang Bi,
  • Haibing Tian,
  • Huali Xue

摘要

The significant health risks associated with ochratoxin A (OTA) contamination in multiple types of foodstuffs underscore the need for detection methods. The inherent complexity of the real sample matrix significantly prolongs OTA analysis time, and the intricate procedural steps present obstacles that must be overcome. Therefore, effective pretreatment is essential. In this study, aptamer-functionalized superparamagnetic chitosan nanoparticles (Apt-SPCO) were synthesized and utilized as adsorbents for the rapid purification and efficient detection of OTA in various real samples, Furthermore, the recognition and binding mechanism between Apt-SPCO and OTA was investigated. Apt-SPCO is spherical in shape, possessing superparamagnetic and anti-interference ability, which can effectively extract and purify OTA in 10 min, and coupled with liquid chromatography (HPLC-FLD) detection method the recovery was 90.9% ~ 106.4% in real sample. Hydrogen bonds were formed between OTA and aptamer (DG-9 bases), with the Kd of 2.72 μM, indicating a high affinity between OTA and aptamer. Meanwhile, Ca2+ induced the aptamer structure changing from a parallel G-quadruplex to an anti-parallel G-quadruplex. These results underscore the user-friendliness, affordable, and excellent selectivity of Apt-SPCO pretreatment. When combined with HPLC-FLD, it exhibits high sensitivity and satisfactory accuracy for OTA determination, particularly in complex food matrix.