Scholarlink

<p>The purpose of this study was to investigate the potential of edible insect extracts from <i>Tenebrio molitor</i> (mealworm) larvae and <i>Bombyx mori</i> (silkworm) larvae and pupae (beondegi) as an alternative to fetal bovine serum (FBS) for culturing stem cell lines of <i>Paralichthys olivaceus</i> (olive flounder). Each extract was prepared using low-temperature extraction at 35&#xa0;°C for 4 h with a solvent consisting of water and ethanol in an 80:20 ratio, followed by concentration under nitrogen gas flow and three rounds of filtration. Several types of commercial serum replacements (KnockOut™, Panexin CD, and CellCor™ CD MSC) were also used for culturing cells. Mealworm extracts successfully replaced up to 85% of FBS when combined with 30% mealworm extract with 70% KnockOut™ or 40% mealworm extract with 60% Panexin CD. Similarly, beondegi extracts effectively replaced up to 85% of FBS, either when using 90% beondegi extract with 10% Panexin CD or alone. These results demonstrate that edible insect extracts, when combined with commercial serum replacements, can replace up to 85% of FBS and offer a feasible alternative for culturing stem cells. The demonstrated reduction in FBS usage indicates potential cost savings in future cultured meat production.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Novel Edible Insect Extracts as a Fetal Bovine Serum Alternative for Culturing Olive Flounder (Paralichthys olivaceus) Stem Cell Lines

  • 期刊论文

  • 发表日期：

    2025年12月17日

查看全文
  • In-Seek Jeong,
  • Hyun-Jin Kim,
  • Jong-Jib Kim,
  • Geon-Woo Park,
  • Young-Min Park,
  • Kyung-Ho Park,
  • Sang-Yun Lee,
  • Sang-Gu Kim

摘要

The purpose of this study was to investigate the potential of edible insect extracts from Tenebrio molitor (mealworm) larvae and Bombyx mori (silkworm) larvae and pupae (beondegi) as an alternative to fetal bovine serum (FBS) for culturing stem cell lines of Paralichthys olivaceus (olive flounder). Each extract was prepared using low-temperature extraction at 35 °C for 4 h with a solvent consisting of water and ethanol in an 80:20 ratio, followed by concentration under nitrogen gas flow and three rounds of filtration. Several types of commercial serum replacements (KnockOut™, Panexin CD, and CellCor™ CD MSC) were also used for culturing cells. Mealworm extracts successfully replaced up to 85% of FBS when combined with 30% mealworm extract with 70% KnockOut™ or 40% mealworm extract with 60% Panexin CD. Similarly, beondegi extracts effectively replaced up to 85% of FBS, either when using 90% beondegi extract with 10% Panexin CD or alone. These results demonstrate that edible insect extracts, when combined with commercial serum replacements, can replace up to 85% of FBS and offer a feasible alternative for culturing stem cells. The demonstrated reduction in FBS usage indicates potential cost savings in future cultured meat production.

Graphical Abstract