Antifungal and Antioxidant Protection of Baccharis dracunculifolia Essential Oil and Nerolidol Nanoparticles Against Fusarium-induced Spoilage in Corn Grains
摘要
Pluronic F-127 nanoparticles (NPs) containing Baccharis dracunculifolia essential oil (NP-EO) or nerolidol (NP-NE) were tested against Fusarium proliferatum, Fusarium verticillioides and Fusarium graminearum. The NPs characterized by dynamic light scattering, zeta potential, differential scanning calorimetry, infrared spectroscopy and scanning electron microscopy resulted in homogeneous particles with average diameters of 219.33 nm (NP-EO) and 372.15 nm (NP-NE). The zeta potential of NP-EO and NP-NE was -18.55 mV and -20.87 mV, respectively. The in vitro assay demonstrated that mycelial growth was significantly inhibited in a dose-dependent response. Depending on the fungal species, the inhibition percentage reached 62–74% for NP-EO and 27–45% for NP-NE. The antifungal activity of NP-EO and NP-NE was confirmed in stored corn kernels, where both formulations caused significant inhibition of Fusarium species. The DPPH radical scavenging activity reached 86% and 63% for NP-EO and NP-NE, respectively, at 140 mg/mL. The antioxidant activity for the ABTS radical assay was near or above 90% for either NP-EO or NP-NE, from a concentration of 50 mg/mL. Additionally, the oxidative stability of corn kernels exposed to bioactive volatiles from the NPs was assessed, revealing a marked reduction in oxidation after 10 days of storage. These findings suggest that bioactive compounds released by the NPs play a key role in fungal inhibition and oxidative damage prevention, supporting their potential application as natural, eco-friendly preservatives in food systems.