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<p>The efficient separation and purification of antioxidant peptides play a pivotal role in the practical application and the exploration of structure–activity relationships. This study employed metal–organic frameworks (MOFs), namely MIL-53(Cr), ZIF-8, and UIO-66, to selectively enrich antioxidant peptides derived from Nile tilapia skins. It was found that the enriched peptides with enhanced antioxidant activity displayed stronger hydrophilicity. Subsequently, UIO-66 was functionalized with the hydrophilic groups, COOH, OH, and NH₂, and the successful modification was validated by a decreased water contact angle. The adsorption behavior was well described by the pseudo-second-order kinetic model and Langmuir thermodynamic model. Notably, 93.37% elution rate of the adsorbed active peptides was achieved. And NH₂-UIO-66 demonstrated the highest enrichment efficiency with significantly increased antioxidant capacity. The IC₅₀ values for scavenging DPPH•, ABTS⁺•, and hydroxyl radicals were 1.02 ± 0.09&#xa0;mg/mL, 2.85 ± 0.16&#xa0;mg/mL, and 2.21 ± 0.15&#xa0;mg/mL, respectively—substantially lower than those of the original tilapia skin hydrolysates. The specific enrichment of antioxidant peptides by modified MOFs was successfully achieved through the size exclusion and hydrophilic interaction. The results in the study offered an innovative approach for peptide separation.</p>

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Selective Enrichment of Antioxidant Peptides from Tilapia Skin by Hydrophilic Modified Metal–Organic Framework Material (UIO-66)

  • 期刊论文

  • 发表日期：

    2025年7月28日

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  • Xingyuan Hu,
  • Yingrou Li,
  • Yue Leng,
  • Huiziyu Guan,
  • Qianwen Sun,
  • Xiangrong Li,
  • Xiang Li,
  • Yidi Cai,
  • Dandan Ren,
  • Long Wu,
  • Hui Zhou

摘要

The efficient separation and purification of antioxidant peptides play a pivotal role in the practical application and the exploration of structure–activity relationships. This study employed metal–organic frameworks (MOFs), namely MIL-53(Cr), ZIF-8, and UIO-66, to selectively enrich antioxidant peptides derived from Nile tilapia skins. It was found that the enriched peptides with enhanced antioxidant activity displayed stronger hydrophilicity. Subsequently, UIO-66 was functionalized with the hydrophilic groups, COOH, OH, and NH₂, and the successful modification was validated by a decreased water contact angle. The adsorption behavior was well described by the pseudo-second-order kinetic model and Langmuir thermodynamic model. Notably, 93.37% elution rate of the adsorbed active peptides was achieved. And NH₂-UIO-66 demonstrated the highest enrichment efficiency with significantly increased antioxidant capacity. The IC₅₀ values for scavenging DPPH•, ABTS⁺•, and hydroxyl radicals were 1.02 ± 0.09 mg/mL, 2.85 ± 0.16 mg/mL, and 2.21 ± 0.15 mg/mL, respectively—substantially lower than those of the original tilapia skin hydrolysates. The specific enrichment of antioxidant peptides by modified MOFs was successfully achieved through the size exclusion and hydrophilic interaction. The results in the study offered an innovative approach for peptide separation.