<p>The study aimed to investigate the impact of an active film composed of oregano essential oil microcapsules, soybean protein isolate (SPI), and sodium carboxymethylcellulose (CMC) (EOMs-SPI-CMC) on the preservation of <i>Agaricus bisporus</i> freshness at 4 ± 1&#xa0;°C. The effectiveness of the prepared active film was evaluated in terms of the physical quality of <i>A. bisporus</i> and regulating the phenylpropane metabolic pathway. The results revealed that the EOMs-SPI-CMC active film effectively preserved the visual appearance and color of <i>A. bisporus</i>, while also maintaining higher moisture content and firmness compared with the control group. Additionally, the active film significantly reduced the respiration rate, electrolyte leakage, and malondialdehyde content, thereby preserving the integrity of the cell membrane and minimizing nutrient loss. The EOMs-SPI-CMC film enhanced the activities of superoxide dismutase and catalase, which suppressed the accumulation of reactive oxygen species and thus reduced oxidative damage and prolonged shelf life. Notably, the active film successfully inhibited enzymatic browning of tissue by reducing the activities of polyphenol oxidase and peroxidase. Furthermore, the active film significantly activated key enzymes associated with the phenylpropane metabolic pathway, resulting in higher retention of phenolic compounds during storage. Collectively, these findings demonstrate that the EOMs-SPI-CMC active film offered a promising approach for extending the shelf life and preserving the postharvest quality of <i>A. bisporus</i>.</p>

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Effect of Active Film Packaging with microencapsulated Oregano Essential Oil on Postharvest Quality and Phenylpropane Metabolic Pathway in Agaricus bisporus

  • Kaiyue Wang,
  • Xiumei Xi,
  • Yifan Wang,
  • Jinhang Lu,
  • Shasha Zhang,
  • Xue Zhou,
  • Xiangyou Wang,
  • Juan Wang

摘要

The study aimed to investigate the impact of an active film composed of oregano essential oil microcapsules, soybean protein isolate (SPI), and sodium carboxymethylcellulose (CMC) (EOMs-SPI-CMC) on the preservation of Agaricus bisporus freshness at 4 ± 1 °C. The effectiveness of the prepared active film was evaluated in terms of the physical quality of A. bisporus and regulating the phenylpropane metabolic pathway. The results revealed that the EOMs-SPI-CMC active film effectively preserved the visual appearance and color of A. bisporus, while also maintaining higher moisture content and firmness compared with the control group. Additionally, the active film significantly reduced the respiration rate, electrolyte leakage, and malondialdehyde content, thereby preserving the integrity of the cell membrane and minimizing nutrient loss. The EOMs-SPI-CMC film enhanced the activities of superoxide dismutase and catalase, which suppressed the accumulation of reactive oxygen species and thus reduced oxidative damage and prolonged shelf life. Notably, the active film successfully inhibited enzymatic browning of tissue by reducing the activities of polyphenol oxidase and peroxidase. Furthermore, the active film significantly activated key enzymes associated with the phenylpropane metabolic pathway, resulting in higher retention of phenolic compounds during storage. Collectively, these findings demonstrate that the EOMs-SPI-CMC active film offered a promising approach for extending the shelf life and preserving the postharvest quality of A. bisporus.