<p>This study investigated the impact of plasma-activated water (PAW) treatment on lentil (<i>Lens culinaris</i>) seed germination, with exposure times of 1 to 15&#xa0;min. PAW generated using an 80% nitrogen and 20% oxygen mixture significantly reduced pH from 7.4 to 2.92 (p &lt; 0.05) and increased nitrate concentration 16-fold after 15&#xa0;min of treatment, while H₂O₂ levels remained unchanged (p &gt; 0.05). Ten minutes of PAW exposure yielded the highest rates and sprouts growth (<i>p</i> &lt; <i>0.05</i>). Protein solubility increased in all PAW-treated samples, peaking at 51% after one minute of treatment (<i>p</i> &lt; <i>0.05</i>). Phytase and α-amylase activities were initially elevated in seeds treated for 1–4&#xa0;min but declined with longer exposures (p &lt; 0.05), likely due to the change in pH. PAW-10 treatment significantly increased GABA content by four-fold than that in untreated lentils (p &lt; 0.05). Electrophoresis revealed similar protein banding patterns (17–170&#xa0;kDa) across samples. FTIR analysis indicated the breakdown of structural components in PAW-treated seeds, except for the antiparallel β-sheet in the control sample. A β-turn was observed in the treated samples, and β-sheet aggregates appeared in the 4-min treatment. Nuclear magnetic resonance (NMR) analysis showed the disappearance of α C₁H and β C₁H anomer signals alongside increased methyl groups. In conclusion, PAW effectively enhances seed germination and nutritional quality, offering a sustainable, innovative method for sprouted seed flour production. However, further research is necessary to optimize industrial scalability and efficiency treatment.</p> Graphical Abstract <p></p>