<p>Climatic changes and coastal pollution emphasize the need for a better understanding of their impact on human health. The present study investigates the impact of coastal pollution on human health by comparing hematological and biochemical parameters and microbial analysis in the coastal population compared to the non-coastal population. A prospective study was conducted involving 508 participants from coastal areas and 92 participants from non-coastal areas. Blood samples were analyzed for hemoglobin, platelet count, renal function, lipid profile, liver function, protein profile, and thyroid function. Feces, urine, and skin swabs were also analyzed for microbial culture analysis. The coastal population showed significant increases in hemoglobin, platelet count, and HDL, while non-coastal participants showed elevated BUN, Serum creatinine, Cholesterol, Triglycerides, and LDL levels. The coastal participants showed a decrease in ALT, AST, Alkaline phosphatase, and levels of thyroid-stimulating hormone. The coastal population had higher rates of hypertension, diabetes, eczema, and other skin lesions, whereas non-coastal participants had higher tinea cases. Microbial analysis revealed differences in fecal, urine, and skin communities, with a higher prevalence of <i>Escherichia coli</i> and other pathogenic bacteria, such as <i>Acinetobacter</i> and <i>Enterobacter</i> species, in coastal samples. The present investigations revealed significant differences in health parameters between coastal and non-coastal participants, which may be attributed to the influence of coastal pollution. Further research with a larger sample size is required to confirm these findings and better understand the impact of coastal pollution on human health.</p>