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<p>The ferritin gene, designated <i>BrFer1</i>, was isolated from the cDNA library of Chinese cabbage leaves treated with sodium nitroprusside (SNP) nitric oxide donor. The <i>BrFer1</i> cDNA encoded a putative polypeptide of 254 amino acids with a molecular mass of 28,183 Da. Its amino acid sequence was highly homologous to several ferritins from other cruciferous plant species like <i>Brassica napus</i>, <i>B. juncea</i> and <i>Arabidopsis thaliana</i>. <i>BrFer1</i> gene expression was rapidly up-regulated in the leaves sprayed by SNP (0.5&#xa0;mM) and gradually increased in dose-dependent. Exogenous FeCl<sub>2</sub> distinctly increased <i>BrFer1</i> expression, whereas FeCl<sub>3</sub> did not affect the gene regulation. <i>BrFer1</i> expression was early responsive to superoxide anion radical generator methyl viologen (MV), but it was hardly induced by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). <i>BrFer1</i> was inducible by FeCl<sub>2</sub>, SNP and MV, but FeCl<sub>2</sub>- and MV-triggered <i>BrFer1</i> gene expression was mediated by iron-mediated signalling. Overexpression of <i>BrFer1</i> in the transgenic Arabidopsis conferred tolerance to MV-triggered oxidative stress. These results suggest that NO-induced <i>BrFer1</i> expression may play a role in dealing with superoxide stress in Chinese cabbage leaves.</p>

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BrFer1 ferritin gene expression during nitric oxide- and superoxide-mediated stress signalling in Chinese cabbage leaves

  • 期刊论文

  • 发表日期：

    2026年3月9日

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  • Yun Jeong Kim,
  • Young Hee Lee,
  • Yu Jin Lee,
  • Juyeoung Um,
  • Hyunsuk Shin,
  • Ryoung Shin,
  • Jeum Kyu Hong

摘要

The ferritin gene, designated BrFer1, was isolated from the cDNA library of Chinese cabbage leaves treated with sodium nitroprusside (SNP) nitric oxide donor. The BrFer1 cDNA encoded a putative polypeptide of 254 amino acids with a molecular mass of 28,183 Da. Its amino acid sequence was highly homologous to several ferritins from other cruciferous plant species like Brassica napus, B. juncea and Arabidopsis thaliana. BrFer1 gene expression was rapidly up-regulated in the leaves sprayed by SNP (0.5 mM) and gradually increased in dose-dependent. Exogenous FeCl2 distinctly increased BrFer1 expression, whereas FeCl3 did not affect the gene regulation. BrFer1 expression was early responsive to superoxide anion radical generator methyl viologen (MV), but it was hardly induced by hydrogen peroxide (H2O2). BrFer1 was inducible by FeCl2, SNP and MV, but FeCl2- and MV-triggered BrFer1 gene expression was mediated by iron-mediated signalling. Overexpression of BrFer1 in the transgenic Arabidopsis conferred tolerance to MV-triggered oxidative stress. These results suggest that NO-induced BrFer1 expression may play a role in dealing with superoxide stress in Chinese cabbage leaves.