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<p>Tocopherols (tocs), potent antioxidants and expression of their biosynthetic genes, improve the plant’s resilience to cope with abiotic stress such as drought and salinity by modulating toc levels based on plant sensitivity and magnitude of the stress. In the present study, overexpression of MPBQ-MT (2-methyl-6-phytyl-1,4-benzoquinol methyltransferase) in soybean drives the synthesis of α and ɤ-toc by modulating the precursor-MPBQ and was functionally validated under simulated drought and salinity stress (PEG (10%), ABA (100&#xa0;µM), and NaCl (250&#xa0;mM)) in <i>E. coli</i> (BL21) and <i>In planta</i>-transformed soybeans through heterologous and transient expression, respectively. Growth of <i>E. coli</i> (BL21) cells harbouring pET29a-MPBQ-MT was significant compared to the controls with vector only under respective stress treatment, validating its cross-species functional role in stress tolerance. With salinity stress, the growth of recombinants was enhanced in spot assay (1.67- and 1.56-fold growth at 10<sup>–3</sup> and 10<sup>–4</sup> dilutions) and liquid-culture assay (1.16-, 1.33-, 1.55-, and 1.85-fold growth at 2, 4, 6, and 8&#xa0;h, respectively). Similarly, overexpressed (OE) soybean lines also showed an augmented toc content, particularly γ-toc (75%, 64.75%, and 69.82%) and α-toc (106%, 100%, and 82.8%) under respective stress treatment compared to wild controls witnessing respective stress. Concurrently, this augmentation improved stress tolerance by enhancing total antioxidant capacity (40.1, 19, and 35.84%), free radical scavenging activity (48.2, 31.6, and 43.82%), and decreasing MDA content (15.3, 18.82, and 10.6%). Additionally, in vivo staining revealed lower H<sub>2</sub>O<sub>2</sub> accumulation in transgenic soybeans than&#xa0;in control plants under stress, highlighting <i>MPBQ-MT’s</i> pivotal role in imparting stress tolerance.</p>

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Expression of MPBQ-methyl transferase confers resistance to simulated drought and salinity tolerance in Glycine max (L.)

  • 期刊论文

  • 发表日期：

    2025年8月13日

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  • Navita Bansal,
  • C. R. Nagesh,
  • G. Rama Prashat,
  • R. Dineshkumar,
  • Ranjeet Ranjan Kumar,
  • Suneha Goswami,
  • Shilpi Aggarwal,
  • Gyan Prakash Mishra,
  • Anil Dahuja,
  • Aruna Tyagi,
  • Shelly Praveen,
  • Rohini Sreevathsa,
  • T. Vinutha

摘要

Tocopherols (tocs), potent antioxidants and expression of their biosynthetic genes, improve the plant’s resilience to cope with abiotic stress such as drought and salinity by modulating toc levels based on plant sensitivity and magnitude of the stress. In the present study, overexpression of MPBQ-MT (2-methyl-6-phytyl-1,4-benzoquinol methyltransferase) in soybean drives the synthesis of α and ɤ-toc by modulating the precursor-MPBQ and was functionally validated under simulated drought and salinity stress (PEG (10%), ABA (100 µM), and NaCl (250 mM)) in E. coli (BL21) and In planta-transformed soybeans through heterologous and transient expression, respectively. Growth of E. coli (BL21) cells harbouring pET29a-MPBQ-MT was significant compared to the controls with vector only under respective stress treatment, validating its cross-species functional role in stress tolerance. With salinity stress, the growth of recombinants was enhanced in spot assay (1.67- and 1.56-fold growth at 10–3 and 10–4 dilutions) and liquid-culture assay (1.16-, 1.33-, 1.55-, and 1.85-fold growth at 2, 4, 6, and 8 h, respectively). Similarly, overexpressed (OE) soybean lines also showed an augmented toc content, particularly γ-toc (75%, 64.75%, and 69.82%) and α-toc (106%, 100%, and 82.8%) under respective stress treatment compared to wild controls witnessing respective stress. Concurrently, this augmentation improved stress tolerance by enhancing total antioxidant capacity (40.1, 19, and 35.84%), free radical scavenging activity (48.2, 31.6, and 43.82%), and decreasing MDA content (15.3, 18.82, and 10.6%). Additionally, in vivo staining revealed lower H2O2 accumulation in transgenic soybeans than in control plants under stress, highlighting MPBQ-MT’s pivotal role in imparting stress tolerance.