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<p>Onion leaf blight, caused by <i>Botrytis squamosa</i>, is a major disease that affects onion leaves, leading to abnormal bulb formation and reduced productivity. Despite its impact, limited information on resistance-related genes in onions hinders the development of effective breeding strategies, highlighting the need for further research to enhance resistance. In this study, the <i>A. cepa lipoxygenase</i> (<i>AcLOX</i>) gene was isolated and functionally characterized using <i>Agrobacterium</i>-mediated transformation in a model plant (<i>Nicotiana tabacum</i>). The <i>AcLOX</i> gene consisted of a single exon (2727&#xa0;bp) and exhibited up to 100-fold higher expression in resistant onions compared to susceptible ones after <i>B. squamosa</i> inoculation. Functional analysis in transgenic tobacco revealed that overexpression lines displayed resistance to <i>B. squamosa</i>, while knockdown lines exhibited more severe symptoms and a faster progression rate. Overexpression plants showed over 110-fold higher <i>AcLOX</i> expression levels, correlating with their resistant phenotype. Conversely, knockdown plants had lower expression than the control, though the difference was not statistically significant. Additionally, Southern blot analysis confirmed stable single-copy T-DNA insertion in transgenic lines. These findings demonstrate that <i>AcLOX</i> overexpression enhances resistance against onion leaf blight and provide fundamental insights for breeding resistant onion varieties and developing environmentally friendly disease management strategies.</p>

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Validation of Allium cepa lipoxygenase (AcLOX) function in enhancing leaf blight resistance in onion

  • 期刊论文

  • 发表日期：

    2025年6月17日

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  • So-Jeong Kim,
  • Young-Doo Park,
  • Jung-Woo Lee

摘要

Onion leaf blight, caused by Botrytis squamosa, is a major disease that affects onion leaves, leading to abnormal bulb formation and reduced productivity. Despite its impact, limited information on resistance-related genes in onions hinders the development of effective breeding strategies, highlighting the need for further research to enhance resistance. In this study, the A. cepa lipoxygenase (AcLOX) gene was isolated and functionally characterized using Agrobacterium-mediated transformation in a model plant (Nicotiana tabacum). The AcLOX gene consisted of a single exon (2727 bp) and exhibited up to 100-fold higher expression in resistant onions compared to susceptible ones after B. squamosa inoculation. Functional analysis in transgenic tobacco revealed that overexpression lines displayed resistance to B. squamosa, while knockdown lines exhibited more severe symptoms and a faster progression rate. Overexpression plants showed over 110-fold higher AcLOX expression levels, correlating with their resistant phenotype. Conversely, knockdown plants had lower expression than the control, though the difference was not statistically significant. Additionally, Southern blot analysis confirmed stable single-copy T-DNA insertion in transgenic lines. These findings demonstrate that AcLOX overexpression enhances resistance against onion leaf blight and provide fundamental insights for breeding resistant onion varieties and developing environmentally friendly disease management strategies.