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<p>Honeysuckle (<i>Lonicera japonica</i> Thunb.), as a traditional Chinese medicinal plant, is well known for its pharmacological properties, especially the high content of chlorogenic acid in honeysuckle. In this study, the cinnamic acid-4-hydroxylase (<i>C4H</i>) gene family in honeysuckle was analyzed based on genome-wide identification, high performance liquid chromatography and real-time fluorescence quantitative detection. A total of 68 <i>C4H</i> genes were identified and named as <i>LjC4H1-68</i>, of which 63 genes were localized to autosomes. The C4H proteins identified varied significantly in length (202 to 757 amino acids) and molecular weight (22.85 kDa to 86.21 kDa), suggesting diverse functional roles. Phylogenetic analyses divided the <i>C4H</i> genes into two subfamilies, indicating different evolutionary trajectories. Gene structure analysis revealed the presence of conserved exons and multiple untranslated regions (UTRs). Conserved motifs identified by MEME software showed specific patterns associated with phylogenetic proximity. Protein–protein interaction network analysis using Arabidopsis thaliana as a reference highlighted C4H31 as a potential hub protein with significant interaction potential. Three-dimensional structural modeling of C4H31 demonstrated functional similarity to cytochrome P450 enzymes, suggesting a possible role in chlorogenic acid biosynthesis and stress responses. This study enhances our understanding of the honeysuckle <i>C4H</i> gene family and its significance for plant development and medicinal applications.</p>

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Genome-wide characterization of the C4H gene family in Honeysuckle: evolution, expression and three-dimensional structural analysis

  • 期刊论文

  • 发表日期：

    2025年7月12日

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  • Xiyao Zeng,
  • Xianjun Long,
  • Yongbo Zhu,
  • Yuming Huang,
  • Hua Yang

摘要

Honeysuckle (Lonicera japonica Thunb.), as a traditional Chinese medicinal plant, is well known for its pharmacological properties, especially the high content of chlorogenic acid in honeysuckle. In this study, the cinnamic acid-4-hydroxylase (C4H) gene family in honeysuckle was analyzed based on genome-wide identification, high performance liquid chromatography and real-time fluorescence quantitative detection. A total of 68 C4H genes were identified and named as LjC4H1-68, of which 63 genes were localized to autosomes. The C4H proteins identified varied significantly in length (202 to 757 amino acids) and molecular weight (22.85 kDa to 86.21 kDa), suggesting diverse functional roles. Phylogenetic analyses divided the C4H genes into two subfamilies, indicating different evolutionary trajectories. Gene structure analysis revealed the presence of conserved exons and multiple untranslated regions (UTRs). Conserved motifs identified by MEME software showed specific patterns associated with phylogenetic proximity. Protein–protein interaction network analysis using Arabidopsis thaliana as a reference highlighted C4H31 as a potential hub protein with significant interaction potential. Three-dimensional structural modeling of C4H31 demonstrated functional similarity to cytochrome P450 enzymes, suggesting a possible role in chlorogenic acid biosynthesis and stress responses. This study enhances our understanding of the honeysuckle C4H gene family and its significance for plant development and medicinal applications.