Development of a highly efficient seeding breeding system of Notopterygium incisum
摘要
The dry root and rhizome of Notopterygium incisum, a traditional Chinese medicine, is used to treat cold symptoms, dispel wind, and dehumidify. However, overharvesting has severely damaged wild populations, threatening their extinction. Seed dormancy and slow growth hinder the supply of cultivated medicinal seedlings and wild population recovery. To address this, we devised an effective breeding system for N. incisum. Optimal seed dormancy breaking involves soaking in 0.50 mg/L BR for 24 h, followed by layering in wet sand under alternating temperatures of 25 °C and 4 °C for 30 days, repeated for 6 months. Disinfected seeds with the inner coat laterally cut are then inoculated into a medium with 1.00 mg/L 6-BA, 2.00 mg/L NAA, and 0.02 mg/L BR, maintained at 16 ± 2 °C. For rapid multiplication, sterile 10 mm × 10 mm leaves are inoculated into D8 media with MS, 2.00 mg/L TDZ, 3.00 mg/L NAA, agar, and sucrose to induce embryonic callus. 30 days later, the embryonic callus is transferred to a fresh medium containing MS, 1.50 mg/L 6-BA, 0.50 mg/L NAA, 0.20 mg/L GA3, 0.50 g/L activated carbon to support the growth and differentiation of embryoid particles. Ultimately, the regenerated plantlets are inoculated into a medium comprising 0.20 mg/L NAA, 8.00 g/L agar, and 30.00 g/L sucrose to induce root development. The proposed system supports the supply of cultivated seedlings, assisting in the conservation and recovery of the wild population of N. incisum.
Graphical abstractIn this work, to improve the breeding efficient of N. incisum, a traditional endangered and precious Chinese medicine, we explored the effect of multiple factors on releasing the dormancy of seeds and established a highly efficient propagation system of N. incisum seeding through somatic embryogenesis using sterile leaf.