<p>To investigate the vibration response of the comprehensive transportation hub structure under multiple-source excitations, an on-site vibration measurement was carried out at Wuhan Railway Station in China. The characteristics of each floor vibration were obtained through the time domain and frequency domain analyses. Based on the vibration characteristic under multiple-source excitations, the proposed attenuation model was derived. In addition, a vibration comfort evaluation on the Wuhan Railway Station was conducted. The results show that the effect of the number of vibration sources on horizontal acceleration is more significant than that regarding vertical acceleration. When the structure is under the effects two vibration sources with different frequencies, a high-frequency vibration can amplify a low-frequency vibration. The derived attenuation model can precisely predict the vibration attenuation and reduce the subsequent vibration test workload. Based on the annoyance rate model result, the annoyance rate of Wuhan Railway Station is high, which is harmful to the staff of the station.</p>