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<p>Cyanobacterial blooms persistently threaten the safety of drinking water. While UV<sub>222</sub> is highly effective and safe for inactivating microorganisms such as viruses and bacteria, its efficacy against cyanobacteria remains unclear. This study investigated the inactivation effects and mechanisms of UV<sub>222</sub> on laboratory-prepared <i>Microcystis aeruginosa</i> (<i>M. aeruginosa</i>) aggregates and unicellular cells. The <i>M. aeruginosa</i> aggregates and unicellular cells could be inactivated by a 1–15 mJ/cm<sup>2</sup> dose of UV<sub>222</sub>, with the inactivation primarily involving damage to the photosystem, cell integrity, and biomacromolecules, along with impaired metabolic functions. The aggregates exhibited greater UV<sub>222</sub> resistance than that of the unicellular cells, attributable to the outer cell shielding and protective protein components exist in extracellular polymeric substances (EPS). Although UV<sub>222</sub> impaired the photosynthetic activity and damaged the cell membranes of the aggregates, their regrowth capacity persisted. Conversely, the unicellular cells suffered extreme inhibition of <i>F</i><sub>v</sub>/<i>F</i><sub>m</sub> and esterase activity, with near-complete loss of regrowth ability at UV<sub>222</sub> doses &gt; 6 mJ/cm<sup>2</sup>. UV<sub>222</sub> directly damaged the cell membrane, chlorophyll-a, and phycocyanin, reducing cyanobacterial activity. Additionally, <i>M. aeruginosa</i> was more readily inactivated during the late lag phase than during the exponential phase (e.g., membrane damage proportions were 95% vs. 87%, respectively). These findings demonstrate the potential of UV<sub>222</sub> for cyanobacterial control and highlight the need to consider practical factors such as aggregation morphology, growth-phase differences, and media effects on radiation efficacy in applications.</p>

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Inactivation and mechanism of UV222 radiation on cyanobacteria: Microcystis aeruginosa in aggregates and unicellular forms

  • 期刊论文

  • 发表日期：

    2026年1月1日

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  • Xuanxuan Xian,
  • Chenlan Chen,
  • Xin Yu,
  • Liang Zhu,
  • Lei Wang,
  • Gang Lian

摘要

Cyanobacterial blooms persistently threaten the safety of drinking water. While UV222 is highly effective and safe for inactivating microorganisms such as viruses and bacteria, its efficacy against cyanobacteria remains unclear. This study investigated the inactivation effects and mechanisms of UV222 on laboratory-prepared Microcystis aeruginosa (M. aeruginosa) aggregates and unicellular cells. The M. aeruginosa aggregates and unicellular cells could be inactivated by a 1–15 mJ/cm2 dose of UV222, with the inactivation primarily involving damage to the photosystem, cell integrity, and biomacromolecules, along with impaired metabolic functions. The aggregates exhibited greater UV222 resistance than that of the unicellular cells, attributable to the outer cell shielding and protective protein components exist in extracellular polymeric substances (EPS). Although UV222 impaired the photosynthetic activity and damaged the cell membranes of the aggregates, their regrowth capacity persisted. Conversely, the unicellular cells suffered extreme inhibition of Fv/Fm and esterase activity, with near-complete loss of regrowth ability at UV222 doses > 6 mJ/cm2. UV222 directly damaged the cell membrane, chlorophyll-a, and phycocyanin, reducing cyanobacterial activity. Additionally, M. aeruginosa was more readily inactivated during the late lag phase than during the exponential phase (e.g., membrane damage proportions were 95% vs. 87%, respectively). These findings demonstrate the potential of UV222 for cyanobacterial control and highlight the need to consider practical factors such as aggregation morphology, growth-phase differences, and media effects on radiation efficacy in applications.