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<p><i>Enterococcus faecium</i>, an opportunistic human pathogen, which shows resistance to multiple antibiotics and imposes serious threats to global public health by causing nosocomial infections. Phage therapy has been reinstated as a substitute of antibiotics for the treatment of multidrug-resistant (MDR) bacterial infections. In this study, we isolated a lytic bacteriophage, SSBHU, against <i>E. faecium</i> ATCC35667 and monitored their lysis efficiency towards multiple clinical strains of <i>E. faecium.</i> Genome of the phage SSBHU composed of linear dsDNA with genome size of 43,953 bps and a GC content of 35.31%, which encodes 75 putative open reading frames without any lysogeny, antibiotic resistance or virulence genes. It belongs to the genus Efemquintavirus, and displayed stable infectivity across different environmental conditions. It has demonstrated antibacterial and antibiofilm properties against both ATCC and clinical strains of <i>E. faecium</i>, possibly due to its broader host range, thereby opening avenues for infection-specific phage therapy. Remarkably, the addition of ribose, trehalose and maltose sugars reduced the bacteriolytic efficiency of the phage SSBHU against an MDR <i>E. faecium</i> strain, but sugar alcohols showed enhanced host lysis by the SSBHU phage. These observations suggested the therapeutic potential of SSBHU phage towards MDR <i>E. faecium</i> infections.</p>

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Isolation and functional characterization of a lytic phage SSBHU against Enterococcus faecium and its potential application in phage therapy

  • 期刊论文

  • 发表日期：

    2026年7月22日

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  • Rakesh Kumar Singh,
  • Ajeet Singh,
  • Riya Anand,
  • Zinnu Rain,
  • Pradyot Prakash,
  • Hari Sharan Misra,
  • Ganesh Kumar Maurya

摘要

Enterococcus faecium, an opportunistic human pathogen, which shows resistance to multiple antibiotics and imposes serious threats to global public health by causing nosocomial infections. Phage therapy has been reinstated as a substitute of antibiotics for the treatment of multidrug-resistant (MDR) bacterial infections. In this study, we isolated a lytic bacteriophage, SSBHU, against E. faecium ATCC35667 and monitored their lysis efficiency towards multiple clinical strains of E. faecium. Genome of the phage SSBHU composed of linear dsDNA with genome size of 43,953 bps and a GC content of 35.31%, which encodes 75 putative open reading frames without any lysogeny, antibiotic resistance or virulence genes. It belongs to the genus Efemquintavirus, and displayed stable infectivity across different environmental conditions. It has demonstrated antibacterial and antibiofilm properties against both ATCC and clinical strains of E. faecium, possibly due to its broader host range, thereby opening avenues for infection-specific phage therapy. Remarkably, the addition of ribose, trehalose and maltose sugars reduced the bacteriolytic efficiency of the phage SSBHU against an MDR E. faecium strain, but sugar alcohols showed enhanced host lysis by the SSBHU phage. These observations suggested the therapeutic potential of SSBHU phage towards MDR E. faecium infections.